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JH295 hydrate 

Cat. No.: HY-116423A
Handling Instructions

JH295 hydrate is a potent, irreversible and selective NIMA-related kinase 2 (Nek2) inhibitor with an IC50 of 770 nM. JH295 hydrate inhibits cellular Nek2 via alkylation of Cys22. JH295 hydrate is inactive against the mitotic kinases, Cdk1, Aurora B or Plk1, and does not perturb bipolar spindle assembly or the spindle assembly checkpoint.

For research use only. We do not sell to patients.

JH295 hydrate Chemical Structure

JH295 hydrate Chemical Structure

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1 mg USD 700 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

JH295 hydrate is a potent, irreversible and selective NIMA-related kinase 2 (Nek2) inhibitor with an IC50 of 770 nM. JH295 hydrate inhibits cellular Nek2 via alkylation of Cys22. JH295 hydrate is inactive against the mitotic kinases, Cdk1, Aurora B or Plk1, and does not perturb bipolar spindle assembly or the spindle assembly checkpoint[1].

IC50 & Target

IC50: 770 nM (Nek2)[1]

In Vitro

JH295 (Compound 16; 0.08-20 μM; 45 minutes; RPMI7951 cells) treatment inhibits WT Nek2 in cells with an IC50 of ~1.3 μM, whereas it has little effect on the C22V mutant[1].

Western Blot Analysis[1]

Cell Line: RPMI7951 cells
Concentration: 0.08 μM, 0.25 μM, 0.74 μM, 2.2 μM, 6.6 μM, 20 μM
Incubation Time: 45 minutes
Result: Inhibited WT Nek2 in cells with an IC50 of ~1.3 μM, whereas had little effect on the C22V mutant.
Molecular Weight

338.36

Formula

C₁₈H₁₈N₄O₃

SMILES

C#CC(NC1=CC2=C(NC(/C2=C\C3=C(C)N=C(CC)N3)=O)C=C1)=O.[H]O[H]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
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Keywords:

JH295JH 295JH-295OthersNek2centrosomalCys22propynamidecellularalkylationcell-permeableInhibitorinhibitorinhibit

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Product Name:
JH295 hydrate
Cat. No.:
HY-116423A
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