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Cat. No.: HY-182495

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Penisimplicissin

Penisimplicissin is a funicone-like compound and antiviral agent. Penisimplicissin reduces the expression of AhR and viral nucleocapsid protein. Penisimplicissin improves the cell viability and morphological characteristics of CCoV-infected cells. Penisimplicissin inhibits CCoV infection. Penisimplicissin can be used in studies related to canine coronavirus infection.

For research use only. We do not sell to patients.

CAS No.: 873207-55-7
Formula: C₁₆H₁₄O₆
Molecular Weight:302.28

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Penisimplicissin Chemical Structure

CAS No.: 873207-55-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

Penisimplicissin (0.1-10 μM; 48 h) exhibits an IC₅₀ of 4.9562 μM in canine fibrosarcoma A72 cells^[1].
Penisimplicissin (0.1-2.5 μM; 48 h) significantly increases the cell viability of canine fibrosarcoma A72 cells infected with canine coronavirus (CCoV) after 48 h of treatment at concentrations of 0.5 μM and 2.5 μM^[1].
Penisimplicissin (0.5 μM) reduces the morphological features of cell death in canine fibrosarcoma A72 cells infected with canine coronavirus (CCoV), including cell shrinkage, pyknosis, and cell detachment^[1].
Penisimplicissin (0.5 μM; 48 h post-infection) significantly reduces viral yield and cytopathic effect in CCoV-infected canine fibrosarcoma A72 cells at 48 h post-treatment^[1].
Penisimplicissin (0.5 μM; 24 h) significantly reduces the expression of aryl hydrocarbon receptor and viral nucleocapsid protein in both uninfected and CCoV-infected canine fibrosarcoma A72 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

canine fibrosarcoma A72 cells
Concentration:

0.1 μM, 0.5 μM, 1 μM, 2.5 μM, 5 μM, 10 μM
Incubation Time:

48 h
Result:

Showed no significant difference in cell viability compared to vehicle control at 0.1, 0.5, and 2.5 μM (p > 0.05).
Significantly reduced cell viability at 1 μM (p < 0.05), 5 μM (p < 0.01), and 10 μM (p < 0.001).
Determined an IC₅₀ of 4.9562 μM in A72 cells.

Cell Viability Assay^[1]

Cell Line:

canine fibrosarcoma A72 cells infected with canine coronavirus (CCoV)
Concentration:

0.1 μM, 0.5 μM, 2.5 μM
Incubation Time:

48 h post-infection
Result:

Showed no significant change in cell viability compared to untreated infected cells at 0.1 μM (p > 0.05).
Significantly increased cell viability in CCoV-infected A72 cells at 0.5 μM (p < 0.01) and 2.5 μM (p < 0.05).
Led to selection of the 0.5 μM dose for subsequent experiments.

Real Time qPCR^[1]

Cell Line:

canine fibrosarcoma A72 cells infected with CCoV
Concentration:

0.5 μM
Incubation Time:

48 h post-infection
Result:

Caused a significant (p < 0.05) decrease in CCoV titer, measured as Log TCID₅₀/mL, compared to untreated infected cells.
Resulted in a decreased cytopathic effect in treated cells relative to untreated infected cells.

Immunofluorescence^[1]

Cell Line:

canine fibrosarcoma A72 cells (uninfected and CCoV-infected)
Concentration:

0.5 μM
Incubation Time:

24 h post-treatment (uninfected cells); 24 h post-infection (infected cells)
Result:

Caused a significant (p < 0.001) reduction in AhR expression in uninfected A72 cells compared to vehicle control.
Resulted in significant reductions in both AhR (p < 0.001) and viral NP (p < 0.001) expression in CCoV-infected A72 cells compared to untreated infected cells.

Chemical Information

CAS No. 873207-55-7
Molecular Weight 302.28
Formula C₁₆H₁₄O₆
SMILES

COC1=C2[C@H](C3=COC(C)=CC3=O)OC(C2=CC(OC)=C1)=O
Structure Classification
- Others
Initial Source
- Microorganisms
Talaromyces pinophilus
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Cerracchio C, et al. In Vitro Evaluation of Antiviral Activities of Funicone-like Compounds Vermistatin and Penisimplicissin against Canine Coronavirus Infection. Antibiotics (Basel). 2023;12(8):1319. Published 2023 Aug 15. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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