CY3-SE
Based on 1 publication(s) in Google Scholar
Cy3-SE (Sulfo-Cy3 NHS ester; Sulfo Cyanine3 NHS ester) is a sulfonated cyanine dye-derived fluorescent labeling reagent with λex of about 515 nm and λem of about 568 nm. Cy3-SE can interact with the π-π stacking of biomolecules (e.g., nucleoside monophosphates, proteins), inhibiting the photoisomerization process and increasing the fluorescence quantum yield and lifetime.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 95.0%
- CAS 番号: 146368-16-3
- 分子式: C35H41N3O10S2
- 分子量:727.84
-
保管条件:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 CY3-SE
More
生物活性
In the presence of nucleoside monophosphates (dNMPs) in solution, Cy3-SE inhibits photoisomerization through π-π stacking interactions, significantly increasing the fluorescence efficiency and lifetime. It has a better enhancement effect on purines (dAMP, dGMP) than on pyrimidines (dCMP, dTMP)[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolution: Dissolve Cy3-SE in DMSO or other suitable organic solvents to prepare a stock solution;
2. Reaction: Dissolve the target molecule (e.g., peptide, antibody) in a buffer solution (e.g., 5 mM PBS, pH 8.0), add an equimolar or excess amount of Cy3-SE solution, and react at room temperature in the dark for 4-6 hours;
3. Purification: Remove unreacted Cy3-SE by dialysis (molecular weight cutoff 2000 Da) or high performance liquid chromatography (HPLC), and collect the labeled product.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
570
550
化学情報
-
CAS 番号 146368-16-3
-
性状 Solid
-
分子量 727.84
-
分子式 C35H41N3O10S2
-
Color Brown to red
-
SMILES
CC(/C(N1CCCCCC(ON2C(CCC2=O)=O)=O)=C\C=C\C3=[N+](CC)C(C=CC(S(=O)([O-])=O)=C4)=C4C3(C)C)(C)C5=C1C=CC(S(=O)(O)=O)=C5
-
別名
Sulfo-Cy3 NHS ester; Sulfo Cyanine3 NHS ester
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Pharmaceutics
The Influence of the External Chemistry of Silica-Based Mesoporous Nanocarriers on Organ Tropism and the Inhibition of Pulmonary Metastases. [Abstract]2025 Oct 26;17(11):1389. PMID: 41304726
溶剤 & 溶解度
DMSO : 50 mg/mL (68.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
-
データシート (280 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
取扱説明書 (2659 KB)
参考文献
[1]. Harvey BJ, et al. Nucleobase-specific enhancement of Cy3 fluorescence. J Fluoresc. 2009 May;19(3):443-8. [Content Brief]
[2]. Dang YQ, et al. Construction of a supramolecular Förster resonance energy transfer system and its application based on the interaction between Cy3-labeled melittin and phosphocholine encapsulated quantum dots. ACS Appl Mater Interfaces. 2012 Mar;4(3):1267-72. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3739 mL | 6.8696 mL | 13.7393 mL | 34.3482 mL |
| 5 mM | 0.2748 mL | 1.3739 mL | 2.7479 mL | 6.8696 mL | |
| 10 mM | 0.1374 mL | 0.6870 mL | 1.3739 mL | 3.4348 mL | |
| 15 mM | 0.0916 mL | 0.4580 mL | 0.9160 mL | 2.2899 mL | |
| 20 mM | 0.0687 mL | 0.3435 mL | 0.6870 mL | 1.7174 mL | |
| 25 mM | 0.0550 mL | 0.2748 mL | 0.5496 mL | 1.3739 mL | |
| 30 mM | 0.0458 mL | 0.2290 mL | 0.4580 mL | 1.1449 mL | |
| 40 mM | 0.0343 mL | 0.1717 mL | 0.3435 mL | 0.8587 mL | |
| 50 mM | 0.0275 mL | 0.1374 mL | 0.2748 mL | 0.6870 mL | |
| 60 mM | 0.0229 mL | 0.1145 mL | 0.2290 mL | 0.5725 mL |
CY3-SE 関連分類
HY-D0823 関連分類
Powered by Bioz