Leucocrystal Violet
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Leucocrystal violet is a cationic triarylmethane dye. Leucocrystal violet generates a colored purple product in the presence of hydrogen peroxide and horseradish peroxidase, or when oxidized by periodate under the catalysis of horseradish peroxidase. Leucocrystal violet serves as a chromogenic reagent for the determination of submicrogram-level hydrogen peroxide, with no interference from tryptophan, persulfate or chromate. Leucocrystal violet reacts with blood to produce a purple/violet color in the presence of hydrogen peroxide; it forms a very weak fluorophore when acting with whole blood in an unbuffered solution, with a maximum absorption wavelength of 630 nm and a maximum fluorescence emission wavelength of 665 nm upon excitation at 630 nm. Leucocrystal violet is widely used for staining blood residues on porous and non-porous materials.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.81%
- CAS 番号: 603-48-5
- 分子式: C25H31N3
- 分子量:373.53
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Leucocrystal violet enables a highly sensitive, precise, and selective cell-free colorimetric assay for hydrogen peroxide, with a molar absorptivity of 75,000 M-1cm-1 for H2O2 detection, stable absorbance readings for at least 4 hours, and minimal interference from tested biological and chemical species[1].
Leucocrystal violet has a weak UV-Vis absorbance maximum at 590 nm; leucocrystal violet working solution has an absorbance maximum at 630 nm due to low pH-induced protonation of crystal violet impurities; unbuffered whole blood mixed with leucocrystal violet working solution produces a strong UV-Vis absorbance maximum at 630 nm[2].
Leucocrystal violet (0.6 g Leucocrystal violet in 450 mL deionized water + 9 g 5-sulfosalicylic acid + 45 mL 30% hydrogen peroxide)-treated blood-borne footwear impressions on dark green polypropylene carpet can be effectively enhanced via spectral methods, with optimal results using 400-600 nm excitation, a 695 nm barrier filter, and an integrated CCD IR colour video camera with image integration; maximum fluorescence intensity occurs at 665 nm emission when excited at 630 nm in the absence of substrate interference[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 603-48-5
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性状 Solid
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分子量 373.53
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分子式 C25H31N3
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Color Blue to blue-green
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SMILES
CN(C)C1=CC=C(C(C2=CC=C(N(C)C)C=C2)C3=CC=C(N(C)C)C=C3)C=C1
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別名
Leucogentian violet; Leucomethyl green; Tris(4-dimethylaminophenyl)methane4,4',4''-Methylidynetris(N,N-dimethylaniline); 4,4′,4″-メチリジントリス(N,N-ジメチルアニリン); ロイコゲンチアンバイオレット; トリス(4-ジメチルアミノフェニル)メタン; ロイコクリスタルバイオレット
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
DMSO : 12.5 mg/mL (33.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (273 KB)
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SDS (458 KB)
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- Italian - IT (458 KB)
- Korean - KR (458 KB)
- Portuguese - PT (458 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6772 mL | 13.3858 mL | 26.7716 mL | 66.9290 mL |
| 5 mM | 0.5354 mL | 2.6772 mL | 5.3543 mL | 13.3858 mL | |
| 10 mM | 0.2677 mL | 1.3386 mL | 2.6772 mL | 6.6929 mL | |
| 15 mM | 0.1785 mL | 0.8924 mL | 1.7848 mL | 4.4619 mL | |
| 20 mM | 0.1339 mL | 0.6693 mL | 1.3386 mL | 3.3465 mL | |
| 25 mM | 0.1071 mL | 0.5354 mL | 1.0709 mL | 2.6772 mL | |
| 30 mM | 0.0892 mL | 0.4462 mL | 0.8924 mL | 2.2310 mL |
Leucocrystal violet 関連分類
HY-D0233 関連分類
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