GCB-27a
GCB-27a is a CD1d-binding immunostimulant and antitumor agent. GCB-27a binds to CD1d to form a stable complex and presents it to NKT cells, enhancing hydrophobic interactions within the A' pocket of CD1d through branched-chain conformation restriction. GCB-27a induces a Th1-biased immune response, drives IFN?γ production and limits IL-4 levels. GCB-27a is applicable to research related to melanoma lung metastasis.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 分子式: C52H95NO10
- 分子量:894.31
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
GCB-27a (1-1000 nM; 72 h) potently stimulates Th1-biased cytokine production in C57BL/6 mouse splenocytes in vitro, with elevated IFN-γ secretion across all tested concentrations and no associated cytotoxicity[1].
GCB-27a (100 nM; 2-24 h) forms more stable and sustained complexes with mouse CD1d on BMDC surfaces than αGalCer, as measured by enhanced and prolonged L363 antibody binding[1].
GCB-27a (24 h) potently induces Th1-biased cytokine production in human NKT cells, with a 2-fold higher frequency of IFN-γ+ cells than αGalCer and comparable IL-4 induction[1].
GCB-27a has stronger binding affinity for both human and murine CD1d than αGalCer, due to optimized hydrophobic interactions and conformational preorganization within the CD1d A' pocket[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
GCB-27a (2.33 nmol per mouse; i.p.; single injection) potently activates innate immune cells in C57BL/6 mice, enhancing DC maturation marker expression and increasing the frequency of IFN-γ-secreting NK cells[1].
GCB-27a (0.58 nmol per mouse; i.p.; single injection) exhibits potent antitumor efficacy in a murine melanoma lung metastasis model, reducing metastatic nodule counts by 89% relative to αGalCer via a sustained Th1-biased immune response[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c mice (n=5)[1]
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Dosage:2.33 nmol per mouse
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Administration:i.p.; single injection
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Result:Induced a 10.9-fold increase in serum IFN-γ levels relative to αGalCer at 24 hours post-injection.
Maintained IL-4 levels comparable to those induced by αGalCer.
Demonstrated the strongest Th1 selectivity among all tested analogs, with the highest IFN-γ/IL-4 ratio fold change over αGalCer.
Confirmed sustained Th1-biased activity via time-dependent cytokine analysis, with peak IFN-γ levels observed at 24 hours post-injection.
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Animal Model:C57BL/6 mice (n=3)[1]
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Dosage:2.33 nmol per mouse
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Administration:i.p.; single injection
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Result:Markedly increased CD80 and CD86 expression on CD11c+ DCs relative to αGalCer.
Elevated the frequency of IFN-γ-producing NK cells to ~20% of splenic NK cells, a significant increase compared to αGalCer.
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Animal Model:C57BL/6 mice (n=6; melanoma lung metastasis model)[1]
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Dosage:0.58 nmol per mouse
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Administration:i.p.; single injection
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Result:Elicited significantly elevated serum IFN-γ levels relative to αGalCer, while IL-4 levels remained comparable to αGalCer.
Achieved an 89% decrease in lung metastatic nodule counts relative to the αGalCer-treated group.
Revealed near-complete inhibition of lung metastasis via histological analysis.
化学情報
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分子量 894.31
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分子式 C52H95NO10
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SMILES
O[C@H]1[C@@H](CO)O[C@H](OC[C@H](NC(CC2=CC=C(OCC(CCCCCCCC)CCCCCCCCCC)C=C2)=O)[C@H](O)[C@H](O)CCCCCCCCCCCCCC)[C@H](O)[C@H]1O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
GCB-27a 関連分類
HY-181739 関連分類
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