Hex-5-ynehydrazide
Based on 1 Customer Validation
Hex-5-ynehydrazide is a bifunctional linker reagent with a terminal hydrazide group and a propargyl group. The hydrazide group can react with an aldehyde to form a semi-permanent hydrozone bond. The propargyl group can be linked to azide-containing biomolecules via Click Chemistry.
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- CAS 番号: 4230-19-7
- 分子式: C6H10N2O
- 分子量:126.16
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
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生物活性
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生物活性
製品説明
化学情報
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CAS 番号 4230-19-7
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分子量 126.16
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分子式 C6H10N2O
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SMILES
O=C(CCCC#C)NN
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
純度とドキュメンテーション
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データシート (237 KB)
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SDS (252 KB)
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取扱説明書 (2659 KB)
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Hex-5-ynehydrazide 関連分類
HY-W840196 関連分類
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