JOE
Based on 1 Customer Validation
JOE is a xanthene fluorophore (i.e., 4′,5′-dichloro-2′,7′-dimethoxy-5 (6)-carboxyfluorescein; 2',7'-dimethoxy-4',5'-dichloro-6-carboxyfluorescein) with an absorption wavelength of approximately 525 nm and an emission wavelength of approximately 550 nm. The fluorescence quantum yield of JOE correlates with the rigidity of the linker arm and the distance to dG nucleoside. JOE is commonly used as a fluorescent label for oligonucleotides and molecular beacon probes, and also serves as the acceptor fluorophore in fluorescence energy transfer primers for DNA sequencing.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 96.36%
- CAS 番号: 82855-40-1
- 分子式: C23H14Cl2O9
- 分子量:505.26
-
保管条件:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
生物活性
The fluorescence quantum yield of JOE (in 1× TBE/7 M urea solution) relative to fluorescein is 0.58, and its emission intensity is lower than that of the F10J ET primer when excited at 488 nm or 514 nm[2].
JOE-labeled molecular beacons exhibit higher fluorescence signals in the hairpin state than in the double-stranded state formed with complementary target ODN, and the signal levels vary with different probe types and linkers[3].
When the JOE-labeled probe forms a hairpin state and a double-stranded state with the complementary target ODN, it exhibits distinct fluorescence melting curve characteristics, thus enabling the differentiation of these two conformations[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
-
CAS 番号 82855-40-1
-
性状 Solid
-
分子量 505.26
-
分子式 C23H14Cl2O9
-
Color Brown to reddish brown
-
SMILES
O=C(C1=CC(C2(C3=C(OC4=C2C=C(OC)C(O)=C4Cl)C(Cl)=C(O)C(OC)=C3)O5)=C(C=C1)C5=O)O
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 50 mg/mL (98.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (9.90 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
-
データシート (281 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
取扱説明書 (2659 KB)
参考文献
[1]. Tsybulsky DA, et al. 4',5'-Dichloro-2',7'-dimethoxy-5(6)-carboxyfluorescein (JOE): synthesis and spectral properties of oligonucleotide conjugates. J Org Chem. 2012;77(2):977-984. [Content Brief]
[2]. Ju J, et al. Fluorescence energy transfer dye-labeled primers for DNA sequencing and analysis. Proc Natl Acad Sci U S A. 1995;92(10):4347-4351. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.9792 mL | 9.8959 mL | 19.7918 mL | 49.4795 mL |
| 5 mM | 0.3958 mL | 1.9792 mL | 3.9584 mL | 9.8959 mL | |
| DMSO | 10 mM | 0.1979 mL | 0.9896 mL | 1.9792 mL | 4.9479 mL |
| 15 mM | 0.1319 mL | 0.6597 mL | 1.3195 mL | 3.2986 mL | |
| 20 mM | 0.0990 mL | 0.4948 mL | 0.9896 mL | 2.4740 mL | |
| 25 mM | 0.0792 mL | 0.3958 mL | 0.7917 mL | 1.9792 mL | |
| 30 mM | 0.0660 mL | 0.3299 mL | 0.6597 mL | 1.6493 mL | |
| 40 mM | 0.0495 mL | 0.2474 mL | 0.4948 mL | 1.2370 mL | |
| 50 mM | 0.0396 mL | 0.1979 mL | 0.3958 mL | 0.9896 mL | |
| 60 mM | 0.0330 mL | 0.1649 mL | 0.3299 mL | 0.8247 mL | |
| 80 mM | 0.0247 mL | 0.1237 mL | 0.2474 mL | 0.6185 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
JOE 関連分類
HY-D1090 関連分類
Powered by Bioz