Natrin
Natrin is a cysteine-rich secretory protein (CRISP) toxin found in snake venom that acts as an ion channel modulator. Natrin blocks the large-conductance calcium-activated potassium channel BKCa with an IC50 of 34.4 nM; it acts as a non-competitive antagonist to inhibit the ryanodine binding capacity and calcium release channel activity of RyR1, with an IC50 of 1.03 μM for purified RyR1. Natrin induces vasoconstrictor responses without affecting basal tension. Natrin induces apoptosis in human hepatoma cells, elevating the Bax/Bcl‑2 ratio through the mitochondrial apoptotic pathway and causing metabolic perturbation. Natrin can be used in research on diseases such as hepatocellular carcinoma.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 69633-04-1
- 分子式: C8H7Cl3O5S
- 分子量:321.56
-
保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
Calcium Channel アイソフォーム固有の製品をすべて表示
More
生物活性
製品説明
|
Bax |
Bcl-2 |
Kv1.3 |
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| SMMC-7721 | IC50 |
49.33 mg/L
|
Cytotoxicity against human SMMC-7721 hepatocellular carcinoma cells assessed as viability reduction incubated 24 hrs by MTT assay.
Cytotoxicity against human SMMC-7721 hepatocellular carcinoma cells assessed as viability reduction incubated 24 hrs by MTT assay.
|
30508545 |
体外実験
Natrin inhibits the binding of [3H]ryanodine to rabbit skeletal muscle SR vesicles with an IC50 of 3.1 µM and decreases Bmax at 3 µM; it inhibits the binding of [3H]ryanodine to purified RyR1 with an IC50 of 1.03 µM and decreases Bmax at 1 µM[3].
Natrin induces contractile responses in endothelium-denuded mouse thoracic aorta and blocks high-conductance calcium-activated potassium channels, the skeletal muscle isoform of ryanodine receptors, and the voltage-gated potassium channel Kv1.3[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:human SMMC-7721 hepatocellular carcinoma cells
-
Concentration:25, 50, 75, 100, 150, 200 mg/L
-
Incubation Time:24 h
-
Result:Inhibited SMMC-7721 proliferation in a concentration-dependent manner.
IC50 = 49.33 mg/L.
-
Cell Line:human SMMC-7721 hepatocellular carcinoma cells
-
Concentration:25, 50 mg/L
-
Incubation Time:24 h
-
Result:Induced apoptotic morphological changes in SMMC-7721 cells.
化学情報
-
CAS 番号 69633-04-1
-
分子量 321.56
-
分子式 C8H7Cl3O5S
-
SMILES
O=S(O)(OCCOC1=CC(Cl)=C(C=C1Cl)Cl)=O
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
[1]. Wang J, et al. Blocking effect and crystal structure of natrin toxin, a cysteine-rich secretory protein from Naja atra venom that targets the BKCa channel. Biochemistry. 2005 Aug 2;44(30):10145-52. [Content Brief]
[3]. Zhou Q, et al. Structural and functional characterization of ryanodine receptor-natrin toxin interaction. Biophysical journal. 2008 Nov 01;95(9):4289-99. [Content Brief]
[4]. Lu S, et al. Metabolomic study of natrin-induced apoptosis in SMMC-7721 hepatocellular carcinoma cells by ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry. International journal of biological macromolecules. 2019 Mar 1;124:1264-73. [Content Brief]
[5]. Averin AS, et al. Cardiovascular Effects of Snake Toxins: Cardiotoxicity and Cardioprotection. Acta naturae. 2021;13(3):4-14. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Natrin 関連分類
HY-187984 関連分類
Powered by Bioz