Nissl Staining Solution (Thionin Method)
Nissl Staining Solution (Thionin Method) is a neural tissue staining solution with thionin as the core component, which stains Nissl bodies purplish blue and nuclear chromatin blue. Nissl Staining Solution (Thionin Method) relies on positively charged thionin molecules to specifically bind to the negatively charged nucleic acid components in the rough endoplasmic reticulum inside Nissl bodies, and achieves differential staining by regulating pH through a buffer system. Nissl Staining Solution (Thionin Method) has pH-dependent staining selectivity; it exhibits the highest staining specificity for neuronal Nissl bodies under pH 3.65 conditions, and can efficiently label glial processes under pH 4.6 conditions.
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
生物活性
製品説明
体外実験
Nissl Staining Solution (Thionin Method) (stain at room temperature for 10 min to overnight) can completely label the chromophilic substances of neurons, with no over-staining occurring throughout the process[1].
Nissl Staining Solution (Thionin Method) exhibits pH-dependent staining selectivity: pH 3.65 enables clear visualization of Nissl bodies, whereas pH 4.6 prominently labels neuroglial cytoplasmic processes[1].
Nissl Staining Solution (Thionin Method) (2-7 min) rapidly labels neuronal somata[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Keywords
- Nissl Staining Solution (Thionin Method)
- Fluorescent Dye
- cell density
- brain region cytoarchitecture
- formalin-fixed nerve cell chromophilic substance
- guinea pig spinal cord sections
- Nissl bodies
- neuronal cell bodies
- human spinal cord sections
- neuronal cytoplasmic components
- neuronal morphology
- glial staining
- Inhibitor
- inhibitor
- inhibit