SBI-183
Based on 1 Customer Validation
SBI-183 is an orally active inhibitor of QSOX1 (Kd: 20 μM). SBI-183 suppresses the proliferative and invasive phenotype of renal cancer cell lines, including triple negative breast cancer cell line, lung adenocarcinoma cell line and pancreatic ductal adenocarcinoma. SBI-183 inhibits tumor growth in two human xenograft mouse models of renal cell carcinoma in vivo .
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.8%
- CAS 番号: 625403-59-0
- 分子式: C18H20N2O2
- 分子量:296.36
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
SBI-183 (6.25-50 μM, 15 min) inhibits the enzymatic activity of rQSOX1 in a dose-dependent manner[1].
SBI-183 inhibits proliferation of tumor cells, but does not kill fibroblasts or rapidly proliferating PBMC (0.625-20 μmol/L, 5 days), inhibits viability of 786-O, RCJ-41T2, and MDA-MB-231 with IC50s of 4.6 μM, 3.9 μM, and 2.4 μM, respectively (0.076 nM-40 μM, 72 h)[1].
SBI-183 (2.5-20 μM, 4-8 days) inhibits tumor invasion in both 2D and 3D models of 786-O, RCJ-41T2, MDA-MB-231, A549 and MIA PaCa2 dose-dependently[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:786-O, RCJ-41T2, MDA-MB-231, A549 和 MIA PaCa2
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Concentration:0.625, 1.25, 2.5, 5, 10, 20 μM
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Incubation Time:5 days
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Result:Inhibited the growth of each tumor cell in a concentration-dependent manner.
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Cell Line:786-O, RCJ-41T2, MDA-MB-231, A549 和 MIA PaCa2
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Concentration:2.5, 5, 10, 20 μM
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Incubation Time:4, 6, 8 days
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Result:Reduced invasion through Matrigel in each cell line.
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Human xenograft mouse models of renal cell carcinoma (786-O and RCJ-41T2)[1]
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Dosage:400 μg/mouse/day (786-O); 100 mg/kg (RCJ-41T2)
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Administration:Oral gavage (p.o.)
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Result:Inhibited the growth of 786-O and RCJ-41T2 in vivo.
Reduced laminin-α4 deposition in RCJ-41T2 mouse xenografts.
化学情報
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CAS 番号 625403-59-0
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性状 Solid
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分子量 296.36
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分子式 C18H20N2O2
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Color White to off-white
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SMILES
O=C(C1=CC(OC)=CC=C1)NC2=CC=C(N3CCCC3)C=C2
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
体外:
DMSO : ≥ 100 mg/mL (337.43 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.44 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.3743 mL | 16.8714 mL | 33.7427 mL | 84.3569 mL |
| 5 mM | 0.6749 mL | 3.3743 mL | 6.7485 mL | 16.8714 mL | |
| 10 mM | 0.3374 mL | 1.6871 mL | 3.3743 mL | 8.4357 mL | |
| 15 mM | 0.2250 mL | 1.1248 mL | 2.2495 mL | 5.6238 mL | |
| 20 mM | 0.1687 mL | 0.8436 mL | 1.6871 mL | 4.2178 mL | |
| 25 mM | 0.1350 mL | 0.6749 mL | 1.3497 mL | 3.3743 mL | |
| 30 mM | 0.1125 mL | 0.5624 mL | 1.1248 mL | 2.8119 mL | |
| 40 mM | 0.0844 mL | 0.4218 mL | 0.8436 mL | 2.1089 mL | |
| 50 mM | 0.0675 mL | 0.3374 mL | 0.6749 mL | 1.6871 mL | |
| 60 mM | 0.0562 mL | 0.2812 mL | 0.5624 mL | 1.4059 mL | |
| 80 mM | 0.0422 mL | 0.2109 mL | 0.4218 mL | 1.0545 mL | |
| 100 mM | 0.0337 mL | 0.1687 mL | 0.3374 mL | 0.8436 mL |
SBI-183 関連分類
HY-165245 関連分類
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