SPAI-1
SPAI-1 is a specific inhibitor for monovalent cation transporting ATPases. SPAI-1 is a peptide isolated from porcine duodenum, inhibits Na+, K+-ATPase and H+, K+-ATPase in vitro, stimulates Mg2+-ATPase.
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- CAS 番号: 131359-77-8
- 分子式: C245H386N72O65S8
- 分子量:5628.57
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
SPAI-1 (10 nM; 2.5 hr) inhibits Na+, K+-ATPase by the competitive mode against Na+ and is uncompetitive with K+. The IC50 of Na+, K+-ATPase inhibition effect is 0.12 μM[1].
SPAI-1 (3-10 μM; 10 or 20 min) significantly inhibits Na+, K+-ATPase[2].
SPAI-1 (0.1-100 μM; 10 or 20 min) shows light difference between enzyme preparations from dog and rat. As isoforms of Na+, K+-ATPase obtained from rats, SPAI-1 shows inhibition with IC50s of 8.7-24.6 μM; as for isoforms of Na+, K+-ATPase obtained from dog, SPAI-1 shows inhibition with IC50s of 11.6-17.5 μM[2].
SPAI-1 (0.1-100 μM; 10 or 20 min) stimulates ouabain-insensitive Mg2+-ATPase at high concentration but failed to inhibit Ca2+-ATPase[2].
SPAI-1 (0.01-50 μM; 10 or 20 min) also inhibits H+, K+-ATPase with an IC50 value of 5 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 131359-77-8
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分子量 5628.57
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分子式 C245H386N72O65S8
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シーケンスの短縮
LLSKRGHCPRILFRCPLSNPSNKCWRDYDCPGVKKCCEGFCGKDCLYPK (Disulfide bridge:Cys8-Cys37,Cys15-Cys41,Cys24-Cys36,Cys30-Cys45)
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
[1]. Araki K, et al. Novel peptide inhibitor (SPAI) of Na+, K+-ATPase from porcine intestine. Biochem Biophys Res Commun. 1989 Oct 16;164(1):496-502. [Content Brief]
[2]. Ishizuka N, et al. Na+,K(+)-ATPase inhibition by an endogenous peptide, SPAI-1, isolated from porcine duodenum. Biochim Biophys Acta. 1991 Nov 4;1069(2):259-66. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
SPAI-1 関連分類
HY-P3711 関連分類
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