TIM-3-IN-2

TIM-3-IN-2 (A-41) binds to purified human TIM-3 protein, and SPR assays show that it has submicromolar affinity with a K_D value of 0.61 μM^[1].
TIM-3-IN-2 binds to purified human TIM-3 protein, and its affinity reaches the submicromolar level as detected by MST, with a K_D of 0.58 μM^[1].
TIM-3-IN-2 (200-1000 nM; 1 h) inhibits the interaction between immobilized human TIM-3 protein and CEACAM1 protein in a competitive ELISA assay^[1].
TIM-3-IN-2 (200-1000 nM) inhibits the interaction between human TIM-3 protein and PtdSer on the surface of apoptotic Jurkat cells in a dose-dependent manner in flow cytometry assays^[1].
TIM-3-IN-2 (1000 nM) inhibits the interaction between human TIM-3 protein and Gal-9 protein in a competitive ELISA assay^[1].
TIM-3-IN-2 (5 μM) restores IFNγ production in human peripheral blood mononuclear cells (PBMCs) that is inhibited by recombinant TIM-3^[1].
TIM-3-IN-2 (5 μM; 0-100 h) reduces the proliferative capacity of NucLight Red-labeled human monocytic AML cells THP-1 in a co-culture system with peripheral blood mononuclear cells (PBMCs), as measured by an Incucyte-based assay^[1].
TIM-3-IN-2 (5 μM; 6 days) enhances the ability of human peripheral blood mononuclear cells (PBMC) to inhibit the proliferation of human acute myeloid leukemia cell line Kasumi-1 in co-culture assays^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.