α-Casein (90-95)
Based on 1 Customer Validation
α-Casein (90-95) is a partial agonist of opioid receptors and a copper ion ligand, with opioid activity. α-Casein (90-95) inhibits the secretion of β-hexosaminidase by rat peritoneal mast cells (PMC) with IC50= 0.1 μM. α-Casein (90-95) inhibits the proliferation of prostate cancer cells LNCaP, DU145, and PC3 with IC50 of 0.94 nM, 137 nM, and 6.92 nM, respectively. α-Casein (90-95) activates Gi-like proteins through a membrane-assisted, receptor-independent pathway, or reversibly binds to opioid receptors, inducing intracellular calcium release and conformational changes, and exerts the activity of promoting mast cell secretion and inhibiting tumor cell proliferation. α-Casein (90-95) can be used in the study of the mechanisms of allergic diseases and prostate cancer.
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- Purity: 99.51%
- CAS No.: 83471-50-5
- 화학식: C38H57N9O9
- 분자량:783.91
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보관:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
All Opioid Receptor Isoforms
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Biological Activity
α-Casein (90-95) (0.1 μM; 10 min) can significantly induce the secretion of β-hexosaminidase from rat peritoneal mast cells (PMC)[2].
α-Casein (90-95) (0.94 nM-137 nM; 4-6 days) inhibits the proliferation of prostate cancer cells (LNCaP, DU145, PC3) in a dose-dependent manner, with an inhibition rate of 30%-59%[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Rat peritoneal mast cells (PMC)
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Concentration:0.1 mM (secretagogue assay), 0.5 mM (BAC inhibition assay)
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Incubation Time:10 min (secretion), 5 min (BAC preincubation)
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Result:Induced 15.2% β-hexosaminidase release (vs. 5.5% spontaneous release) at 0.1 mM, with maximal secretion within 1 min.
Preincubation with 3.0 μg/mL Benzalkonium chloride (HY-B2232) abolished secretion (inhibition 50.1%), indicating membrane-mediated Gi-protein activation.
Chemical Information
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CAS No. 83471-50-5
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Appearance Solid
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분자량 783.91
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화학식 C38H57N9O9
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Color White to off-white
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Sequence
Arg-Tyr-Leu-Gly-Tyr-Leu
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Sequence Shortening
RYLGYL
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
용액&용해도
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
| Contents | Assign value | |
| Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 |
| Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 |
| Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution:
| Overall charge of peptide | Details |
| Negative (<0) |
1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. |
| Positive (>0) |
1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. |
| Zero (=0) |
1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
순도&문서
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Chruścińska E, et al. Binding ability of Cu2+ ions by opiate-like fragments of bovine casein. J Inorg Biochem. 1997 Apr;66(1):19-22. [Content Brief]
[3]. Kampa M, et al. Opioid alkaloids and casomorphin peptides decrease the proliferation of prostatic cancer cell lines (LNCaP, PC3 and DU145) through a partial interaction with opioid receptors. Eur J Pharmacol. 1997 Sep 24;335(2-3):255-65. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
α-Casein (90-95) Related Classifications
HY-P1793 Related Classifications
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