α-Casein (90-95) TFA
Based on 1 Customer Validation
α-Casein (90-95) TFA is a partial agonist of opioid receptors and a copper ion ligand, with opioid activity. α-Casein (90-95) TFA inhibits the secretion of β-hexosaminidase by rat peritoneal mast cells (PMC) with IC50= 0.1 μM. α-Casein (90-95) TFA inhibits the proliferation of prostate cancer cells LNCaP, DU145, and PC3 with IC50 of 0.94 nM, 137 nM, and 6.92 nM, respectively. α-Casein (90-95) TFA activates Gi-like proteins through a membrane-assisted, receptor-independent pathway, or reversibly binds to opioid receptors, inducing intracellular calcium release and conformational changes, and exerts the activity of promoting mast cell secretion and inhibiting tumor cell proliferation. α-Casein (90-95) TFA can be used in the study of the mechanisms of allergic diseases and prostate cancer.
For research use only. We do not sell to patients.
- Purity: 99.58%
- Formula: C40H58F3N9O11
- Molecular Weight:897.94
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
α-Casein (90-95) TFA (0.1 μM; 10 min) can significantly induce the secretion of β-hexosaminidase from rat peritoneal mast cells (PMC)[2].
α-Casein (90-95) TFA (0.94 nM-137 nM; 4-6 days) inhibits the proliferation of prostate cancer cells (LNCaP, DU145, PC3) in a dose-dependent manner, with an inhibition rate of 30%-59%[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Rat peritoneal mast cells (PMC)
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Concentration:0.1 mM (secretagogue assay), 0.5 mM (BAC inhibition assay)
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Incubation Time:10 min (secretion), 5 min (BAC preincubation)
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Result:Induced 15.2% β-hexosaminidase release (vs. 5.5% spontaneous release) at 0.1 mM, with maximal secretion within 1 min.
Preincubation with 3.0 μg/mL Benzalkonium chloride (HY-B2232) abolished secretion (inhibition 50.1%), indicating membrane-mediated Gi-protein activation.
Chemical Information
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Appearance Solid
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Molecular Weight 897.94
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Formula C40H58F3N9O11
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Color White to off-white
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Sequence
Arg-Tyr-Leu-Gly-Tyr-Leu
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Sequence Shortening
RYLGYL
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Solvent & Solubility
H2O : ≥ 100 mg/mL (111.37 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Chruścińska E, et al. Binding ability of Cu2+ ions by opiate-like fragments of bovine casein. J Inorg Biochem. 1997 Apr;66(1):19-22. [Content Brief]
[3]. Kampa M, et al. Opioid alkaloids and casomorphin peptides decrease the proliferation of prostatic cancer cell lines (LNCaP, PC3 and DU145) through a partial interaction with opioid receptors. Eur J Pharmacol. 1997 Sep 24;335(2-3):255-65. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.1137 mL | 5.5683 mL | 11.1366 mL | 27.8415 mL |
| 5 mM | 0.2227 mL | 1.1137 mL | 2.2273 mL | 5.5683 mL | |
| 10 mM | 0.1114 mL | 0.5568 mL | 1.1137 mL | 2.7842 mL | |
| 15 mM | 0.0742 mL | 0.3712 mL | 0.7424 mL | 1.8561 mL | |
| 20 mM | 0.0557 mL | 0.2784 mL | 0.5568 mL | 1.3921 mL | |
| 25 mM | 0.0445 mL | 0.2227 mL | 0.4455 mL | 1.1137 mL | |
| 30 mM | 0.0371 mL | 0.1856 mL | 0.3712 mL | 0.9281 mL | |
| 40 mM | 0.0278 mL | 0.1392 mL | 0.2784 mL | 0.6960 mL | |
| 50 mM | 0.0223 mL | 0.1114 mL | 0.2227 mL | 0.5568 mL | |
| 60 mM | 0.0186 mL | 0.0928 mL | 0.1856 mL | 0.4640 mL | |
| 80 mM | 0.0139 mL | 0.0696 mL | 0.1392 mL | 0.3480 mL | |
| 100 mM | 0.0111 mL | 0.0557 mL | 0.1114 mL | 0.2784 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
α-Casein (90-95) TFA Related Classifications
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