Celltrack Deep Red

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Dye preparation
Stock solution: Dissolve in DMSO to a final concentration of 1 mM (protect from light and store in aliquots).
Working solution: Dilute the stock solution to a working concentration of 250 nM-1 μM in serum-free medium. Preheat the staining working solution to 37 °C.

2. Cell labeling

2.1 Staining protocol for cells in suspension
2.1.1 Harvest cells by centrifugation and aspirate the supernatant. Resuspend the cells gently in pre-warmed Celltrack Deep Red Working Solution.
2.1.2 Incubate 15-45 minutes under growth conditions appropriate for the particular cell type.
2.1.3 Centrifuge the cells and remove the the dye.
2.1.4 Change to fresh culture medium.
2.1.5 Fluorescence microscopy: Ex/Em = 630/650 nm.

2.2 Staining protocol for adherent cells
2.2.1 Remove culture media, and gently add the pre-warmed Celltrack Deep Red Working Solution.
2.2.2 Incubate 15-45 minutes under growth conditions appropriate for the particular cell type.
2.2.3 Remove the Celltrack Deep Red Working Solution.
2.2.4 Change to fresh culture medium.
2.2.5 Fluorescence microscopy: Ex/Em = 630/650 nm.

Notes
1. Please adjust the Celltrack Deep Red working solution concentration, incubation temperature and time according to actual conditions.
2. To maintain normal cellular physiology and reduce potential artifacts, keep the dye concentration as low as possible.
3. Avoid using buffers containing amino or thiol groups.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.