Deoxymorellin
Deoxymorellin is a caged xanthone found in resins and fruits of Garcinia hanburyi hook. f.. Deoxymorellin exhibits cytotoxicity. Deoxymorellin can be used for research of Methicillin (HY-121544)-resistant or Methicillin-susceptible Staphylococcus aureus.
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- CAS No.: 1064-34-2
- 화학식: C33H38O6
- 분자량:530.65
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
In Vitro
Deoxymorellin (30 μg) does not inhibit the growth of MSSA ATCC 25923 or MRSA USA300 strain SF8300 in a disk diffusion assay at 30 μg per disk[1].
Deoxymorellin exhibits potent cytotoxicity against mammalian cells with an IC50 of 1.42 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1064-34-2
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분자량 530.65
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화학식 C33H38O6
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SMILES
C/C(C)=C\C[C@@]12[C@]34C(C(C5=C(O)C(C=CC(C)(O6)C)=C6C(C/C=C(C)\C)=C5O3)=O)=C[C@@](C[C@@]4([H])C(C)(O2)C)([H])C1=O
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocol
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
순도&문서
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Deoxymorellin Related Classifications
HY-182461 Related Classifications
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