Dopal
Dopal is a metabolite and aldehyde neurotoxin of Dopamine (HY-B0451). Dopal exhibits cytotoxicity, induces α-synuclein oligomerization and aggregation, and is closely associated with the pathogenesis of Parkinson's disease. Dopal can be used in research related to Parkinson's disease.
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- Purity: 90.0%
- CAS No.: 5707-55-1
- 화학식: C8H8O3
- 분자량:152.15
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보관:
-80°C
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Biological Activity
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Chemical Information
- 순도&문서
- References
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Help & FAQs
All α-synuclein Isoforms
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Biological Activity
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α-synuclein Aggregation |
Dopal (1.5-1500 μM; 0-4 h) induces dose- and time-dependent aggregation of purified human recombinant α-synuclein in a cell-free system; aggregation initiates at concentrations as low as 1.5 μM, and large polymeric α-synuclein aggregates form at concentrations of 300 μM and above[1].
Dopal (3 μM-1 mM; 2-8 h) induces dose-dependent α-synuclein aggregation and cytotoxicity in SHSY-5Y cells overexpressing wild-type α-synuclein, with significant aggregation observed at concentrations ≥30 μM, oligomer formation initiated at 3 μM, and rapid cell death occurring at 1 mM[1].
Dopal (100-150 μM; 18-24 h) exhibits toxicity to SH-SY5Y cells and also induces cell apoptosis[2].
Dopal (100-200 μM; 16-24 h) induces the formation of α-synuclein-EGFP dimers in SH-SY5Y cells overexpressing α-synuclein-EGFP[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SH-SY5Y neuroblastoma cells
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Concentration:100 μM
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Incubation Time:24 h
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Result:Induced ~30% death in the SH-SY5Y cell population, significantly reducing cell viability compared to untreated controls.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague-Dawley (male, 2-month-old, 300 g, unilateral stereotactic injection into substantia nigra)[1]
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Dosage:1.0 μg; 0.2 μg
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Administration:stereotactic injection into substantia nigra; single dose
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Result:Increased 50 to 170 kDa α-synuclein (AS) oligomers by 2.75-fold relative to vehicle control, with these oligomers accounting for 63% of total AS at 4 hours post-injection with 1.0 μg dose.
Induced a lesser increase in 50 to 170 kDa AS oligomers at 4 hours post-injection with 0.2 μg dose compared to 1.0 μg dose.
Caused loss of tyrosine hydroxylase immunoreactivity in the substantia nigra at 24 and 48 hours post-injection with both 1.0 μg and 0.2 μg doses. Failed to detect large AS aggregates via immunohistochemistry at 4, 24, or 48 hours post-injection.
Chemical Information
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CAS No. 5707-55-1
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Appearance Liquid
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분자량 152.15
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화학식 C8H8O3
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Color Colorless to light yellow
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SMILES
O=CCC1=CC=C(O)C(O)=C1
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선적
Shipping with dry ice.
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보관
-80°C
순도&문서
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Burke WJ, et al. Aggregation of alpha-synuclein by DOPAL, the monoamine oxidase metabolite of dopamine. Acta Neuropathol. 2008;115(2):193-203. [Content Brief]
[2]. Bloch DN, et al. Scavenging neurotoxic aldehydes using lysine carbon dots. Nanoscale Adv. 2023;5(5):1356-1367. Published 2023 Jan 19. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Dopal Related Classifications
HY-121252 Related Classifications
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