Fluo-3FF AM

Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
1. Preparation of Fluo-3FF AM Solution 1.1 Preparation of Storage Solution Prepare a 1 mM stock solution by diluting Fluo-3FF AM with a 0.025% (w/v) Pluronic F-127/dimethyl sulfoxide solution.
1.2 Preparation of Working Solution Prepare a 5 μM working solution of Fluo-3 AM using a balanced buffer solution.
Note: Please adjust the concentration of the Fluo-3FF AM working solution according to the actual situation, and prepare it as needed.
2. Cell Staining 2.1 Cultivate adherent cells on sterile coverslips.
2.2 Remove the cover glass from the culture medium and aspirate away the excess medium.
2.3 Add 100 μL of the dye working solution and gently shake to ensure complete coverage of the cells. Incubate for 60 minutes.
a) Remove the dye working solution and then wash it in PSS at 4 °C for 60 minutes.
b) Imaging/Detection: The excitation wavelength is approximately between 462 - 506 nm, and the emission signal is detected at approximately 526 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.