OXFBD02
Based on 1 Customer Validation
OXF BD 02 is a selective inhibitor of BRD4(1) (the first bromodomain of BRD4) with IC50 value of 382 nM.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 98.04%
- CAS No.: 1429129-68-9
- 화학식: C18H17NO3
- 분자량:295.33
-
보관:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Biological Activity
|
BRD4(1) 382 nM (IC50) |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| 786-0 | GI50 |
27.1 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human 786-0 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human 786-0 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| A498 | GI50 |
1.58 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human A498 cells after 48 hrs by SRB assay
Growth inhibition of human A498 cells after 48 hrs by SRB assay
|
[PMID: 29776834] |
| A549 | GI50 |
22.4 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human A549/ATCC cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human A549/ATCC cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| A549 | IC50 |
>10 μM
Compound: 8
|
Cytotoxicity against human A549 cells after 72 hrs by MTS assay
Cytotoxicity against human A549 cells after 72 hrs by MTS assay
|
[PMID: 23517011] |
| ACHN | GI50 |
4.76 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human ACHN cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human ACHN cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| BT-549 | GI50 |
28.2 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human BT549 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human BT549 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| CAKI-1 | GI50 |
4.58 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human CAKI-1 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human CAKI-1 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| CCRF-CEM | GI50 |
14.8 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human CCRF-CEM cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human CCRF-CEM cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| COLO 205 | GI50 |
5.25 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human COLO205 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human COLO205 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| DU-145 | GI50 |
6.95 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human DU145 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human DU145 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| HCC 2998 | GI50 |
8.55 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human HCC2998 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human HCC2998 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| HCT-116 | GI50 |
7.08 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human HCT116 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human HCT116 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| HCT-15 | GI50 |
3.52 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human HCT15 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human HCT15 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| HeLa | IC50 |
>100 μM
Compound: 8
|
Cytotoxicity against human HeLa cells after 24 hrs by WST-1 assay
Cytotoxicity against human HeLa cells after 24 hrs by WST-1 assay
|
[PMID: 23517011] |
| HL-60(TB) | GI50 |
2.17 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human HL-60(TB) cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human HL-60(TB) cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| HOP-62 | GI50 |
4.89 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human HOP62 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human HOP62 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| HOP-92 | GI50 |
1.02 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human HOP92 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human HOP92 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| Hs-578T | GI50 |
1.93 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human Hs 578T cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human Hs 578T cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| HT-29 | GI50 |
4.54 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human HT-29 cells after 48 hrs by SRB assay
Growth inhibition of human HT-29 cells after 48 hrs by SRB assay
|
[PMID: 29776834] |
| IGROV-1 | GI50 |
23.2 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human IGROV1 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human IGROV1 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| K562 | GI50 |
8.69 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human K562 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human K562 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| KM12 | GI50 |
8.2 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human KM12 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human KM12 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| LOX IMVI | GI50 |
3.6 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human LOXIMVI cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human LOXIMVI cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| M14 | GI50 |
4.48 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human M14 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human M14 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| Malme-3M | GI50 |
28.9 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human MALME-3M cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human MALME-3M cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| MCF7 | GI50 |
1.25 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human MCF7 cells after 48 hrs by SRB assay
Growth inhibition of human MCF7 cells after 48 hrs by SRB assay
|
[PMID: 29776834] |
| MDA-MB-231 | GI50 |
3.92 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human MDA-MB-231 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human MDA-MB-231 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| MDA-MB-435 | GI50 |
3.48 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human MDA-MB-435 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human MDA-MB-435 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| MDA-MB-468 | GI50 |
1.89 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human MDA-MB-468 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human MDA-MB-468 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| MOLT-4 | GI50 |
17.9 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human MOLT4 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human MOLT4 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| MV4-11 | IC50 |
0.794 μM
Compound: 8
|
Cytotoxicity against human MV4-11 cells after 72 hrs by MTS assay
Cytotoxicity against human MV4-11 cells after 72 hrs by MTS assay
|
[PMID: 23517011] |
| NCI/ADR-RES | GI50 |
6.32 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human NCI-ADR-RES cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human NCI-ADR-RES cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| NCI-H1975 | IC50 |
>10 μM
Compound: 8
|
Cytotoxicity against human NCI-H1975 cells after 72 hrs by MTS assay
Cytotoxicity against human NCI-H1975 cells after 72 hrs by MTS assay
|
[PMID: 23517011] |
| NCI-H226 | GI50 |
21.3 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human NCI-H226 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human NCI-H226 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| NCI-H322M | GI50 |
8.41 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human NCI-H322M cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human NCI-H322M cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| NCI-H460 | GI50 |
25.1 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human NCI-H460 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human NCI-H460 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| NCI-H522 | GI50 |
27 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human NCI-H522 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human NCI-H522 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| OVCAR-3 | GI50 |
6.64 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human OVCAR3 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human OVCAR3 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| OVCAR-4 | GI50 |
6.35 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human OVCAR4 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human OVCAR4 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| OVCAR-5 | GI50 |
31.8 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human OVCAR5 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human OVCAR5 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| OVCAR-8 | GI50 |
8.33 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human OVCAR8 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human OVCAR8 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| RPMI-8226 | GI50 |
1.94 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human RPMI8226 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human RPMI8226 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| RXF 393 | GI50 |
17.2 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human RXF393 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human RXF393 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| SF-268 | GI50 |
39.3 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human SF268 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SF268 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| SF-295 | GI50 |
7.31 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human SF295 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SF295 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| SF-539 | GI50 |
4.56 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human SF539 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SF539 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| SK-MEL-2 | GI50 |
23.5 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human SK-MEL-2 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SK-MEL-2 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| SK-MEL-28 | GI50 |
9.15 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human SK-MEL-28 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SK-MEL-28 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| SK-MEL-5 | GI50 |
5.95 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human SK-MEL-5 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SK-MEL-5 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| SK-OV-3 | GI50 |
7.01 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human SKOV3 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SKOV3 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| SN12C | GI50 |
3.99 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human SN12C cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SN12C cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| SNB-19 | GI50 |
5.69 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human SNB19 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SNB19 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| SNB-75 | GI50 |
0.423 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human SNB75 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SNB75 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| SR | GI50 |
1.01 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human SR cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SR cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| SW-620 | GI50 |
24.5 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human SW620 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SW620 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| T47D | GI50 |
7.7 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human T47D cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human T47D cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| TK-10 | GI50 |
1.08 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human TK10 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human TK10 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| U-251 | GI50 |
15 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human U251 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human U251 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| U2OS | IC50 |
>100 μM
Compound: 8
|
Cytotoxicity against human U2OS cells after 24 hrs by WST-1 assay
Cytotoxicity against human U2OS cells after 24 hrs by WST-1 assay
|
[PMID: 23517011] |
| UACC-257 | GI50 |
4.66 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human UACC257 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human UACC257 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
| UO-31 | GI50 |
4.58 μM
Compound: 1a; OXFBD02
|
Growth inhibition of human UO31 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human UO31 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 29776834] |
Chemical Information
-
CAS No. 1429129-68-9
-
Appearance Solid
-
분자량 295.33
-
화학식 C18H17NO3
-
Color White to off-white
-
SMILES
OC(C1=CC=CC=C1)C2=CC(O)=CC(C3=C(C)ON=C3C)=C2
-
선적
Room temperature in continental US; may vary elsewhere.
-
보관
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
용액&용해도
DMSO : 100 mg/mL (338.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.47 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.04 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3860 mL | 16.9302 mL | 33.8604 mL | 84.6511 mL |
| 5 mM | 0.6772 mL | 3.3860 mL | 6.7721 mL | 16.9302 mL | |
| 10 mM | 0.3386 mL | 1.6930 mL | 3.3860 mL | 8.4651 mL | |
| 15 mM | 0.2257 mL | 1.1287 mL | 2.2574 mL | 5.6434 mL | |
| 20 mM | 0.1693 mL | 0.8465 mL | 1.6930 mL | 4.2326 mL | |
| 25 mM | 0.1354 mL | 0.6772 mL | 1.3544 mL | 3.3860 mL | |
| 30 mM | 0.1129 mL | 0.5643 mL | 1.1287 mL | 2.8217 mL | |
| 40 mM | 0.0847 mL | 0.4233 mL | 0.8465 mL | 2.1163 mL | |
| 50 mM | 0.0677 mL | 0.3386 mL | 0.6772 mL | 1.6930 mL | |
| 60 mM | 0.0564 mL | 0.2822 mL | 0.5643 mL | 1.4109 mL | |
| 80 mM | 0.0423 mL | 0.2116 mL | 0.4233 mL | 1.0581 mL | |
| 100 mM | 0.0339 mL | 0.1693 mL | 0.3386 mL | 0.8465 mL |