Phthalaldehyde

Phthalaldehyde (0.0025% (w/v); 1-180 min) reacts with lysine, glycine, and histidine to produce distinct UV absorption peaks at both acidic and alkaline pH, with reaction rates accelerating at higher pH; specific peak patterns and development times vary by amino acid^[1].
Phthalaldehyde (0.05-0.2% (w/v); 2 h at 20 °C) induces dark color changes in pellets of Gram-negative (E. coli, P. aeruginosa) and Gram-positive (S. aureus) bacteria, with darker colors observed at alkaline pH and higher OPA concentration^[1].
Phthalaldehyde (0.1% (w/v); 30 min at 20 °C) significantly inhibits EDTA-Tris induced lysis of Pseudomonas aeruginosa PA01^[1].
Phthalaldehyde (0.1% (w/v); 30 min at 20 °C) significantly inhibits lysostaphin-induced lysis of Staphylococcus aureus NCTC 6571, with efficacy comparable to glutaraldehyde^[1].
Phthalaldehyde (0.25-0.5% (w/v); 60 min) significantly increases the absorbance of Escherichia coli ATCC 9481, Staphylococcus aureus NCTC 6571, and Pseudomonas aeruginosa PA01 suspensions, with a greater increase in E. coli absorbance observed at the 0.5% (w/v) concentration^[1].
Phthalaldehyde detects the blocked amino-terminal tryptic peptide of rat liver acyl-CoA oxidase by forming a fluorescent Schiff base with the peptide's free α-amino group^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.