SppIP
Based on 1 Customer Validation
SppIP (Sakacin P inducer peptide) is a peptide consisting of 19 amino acids. SppIP is an essential inducer for sakacin P production. SppIP can induce the expression of PRRSV GP5 and SARS-CoV-2 spike protein in recombinant Lactobacillus plantarum. SppIP can activate the transcription of the sakacin P promoter to drive the expression of downstream heterologous capsid proteins, increasing the proportion of PCV2d capsid proteins displayed on the cell surface of Lactobacillus plantarum. SppIP can be used in studies related to sakacin P induction.
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- Purity: 98.99%
- CAS No.: 175483-64-4
- 화학식: C99H157N31O26S
- 분자량:2229.56
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보관:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
SppIP (10-200 ng/mL; 4-8 h) induces expression of PRRSV GP5 in recombinant Lactobacillus plantarum CGMCC 1.557, with maximum production achieved using 200 ng/mL SppIP for 7 h at 33 °C in the pSIP-1320-O5MH strain, and expression remains stable across 5 bacterial passages[2].
SppIP (50 ng/mL; 8 h) induces expression of SARS-CoV-2 spike protein in Lactobacillus plantarum Lp18:S that is stable after 20 min at 50 °C or 30 min at pH = 1.5, and shows increased expression after 2 h exposure to 0.2% bile salt[3].
SppIP (50 ng/mL; 3-12 h) induces time-dependent expression and surface localization of PCV2d His6-tag-capsid protein in recombinant Lactobacillus plantarum pLp3050-His6-tag-capsid, with maximal production (4.8 μg per 1010 CFU) in cell wall fractions after 12 h, while also reducing bacterial growth rate[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:recombinant Lactobacillus plantarum Lp18:S strain
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Concentration:50 ng/mL
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Incubation Time:8 h
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Result:Induced expression of SARS-CoV-2 spike protein.
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Cell Line:recombinant Lactobacillus plantarum pLp3050-His6-tag-capsid
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Concentration:50 ng/mL
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Incubation Time:3 h
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Result:Detected ~25 kDa His6-tag-capsid protein in cell-free supernatants and ~28 kDa protein (signal peptide + His6-tag-capsid) in cell wall fractions of recombinant L.
plantarum after 3 h induction; no such bands were seen in non-induced cells or control L.
plantarum pLp3050 cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c (female, 6-week-old)[4]
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Dosage:50 ng/mL (added to bacterial cultures for induction)
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Administration:in vitro induction; 12 h
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Result:Produced 4.8 μg PCV2d capsid protein per 1010 CFU of recombinant L.
plantarum in the cell wall fraction.
Produced 1.6 μg PCV2d capsid protein per 1010 CFU of recombinant L.
plantarum in the cell-free supernatant.
Elicited a significant increase in fecal capsid-specific sIgA levels.
Induced fecal virus neutralizing activity with a 50% reduction in PCV2d infectivity at a dilution factor of 1:55.
Chemical Information
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CAS No. 175483-64-4
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Appearance Solid
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분자량 2229.56
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화학식 C99H157N31O26S
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Color White to off-white
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Synonyms
Sakacin P inducer peptide
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Sequence
Met-Ala-Gly-Asn-Ser-Ser-Asn-Phe-Ile-His-Lys-Ile-Lys-Gln-Ile-Phe-Thr-His-Arg
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Sequence Shortening
MAGNSSNFIHKIKQIFTHR
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
용액&용해도
DMSO : 100 mg/mL (44.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 7.14 mg/mL (3.20 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Eijsink VG, et al. Induction of bacteriocin production in Lactobacillus sake by a secreted peptide. J Bacteriol. 1996;178(8):2232-2237. [Content Brief]
[2]. Wang M, et al. Construction and optimization of Lactobacillus plantarum expression system expressing glycoprotein 5 of porcine reproductive and respiratory syndrome virus. Int J Biol Macromol. 2020 Jan 15;143:112-117. [Content Brief]
[3]. Wang M, et al. A recombinant Lactobacillus plantarum strain expressing the spike protein of SARS-CoV-2. Int J Biol Macromol. 2020;160:736-740. [Content Brief]
[4]. Tseng YH, et al. Construction of a Lactobacillus plantarum Strain Expressing the Capsid Protein of Porcine Circovirus Type 2d (PCV2d) as an Oral Vaccine. Indian J Microbiol. 2019 Dec;59(4):490-499. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 0.4485 mL | 2.2426 mL | 4.4852 mL | 11.2130 mL |
| DMSO | 5 mM | 0.0897 mL | 0.4485 mL | 0.8970 mL | 2.2426 mL |
| 10 mM | 0.0449 mL | 0.2243 mL | 0.4485 mL | 1.1213 mL | |
| 15 mM | 0.0299 mL | 0.1495 mL | 0.2990 mL | 0.7475 mL | |
| 20 mM | 0.0224 mL | 0.1121 mL | 0.2243 mL | 0.5606 mL | |
| 25 mM | 0.0179 mL | 0.0897 mL | 0.1794 mL | 0.4485 mL | |
| 30 mM | 0.0150 mL | 0.0748 mL | 0.1495 mL | 0.3738 mL | |
| 40 mM | 0.0112 mL | 0.0561 mL | 0.1121 mL | 0.2803 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
SppIP Related Classifications
HY-P11146 Related Classifications
Powered by Bioz
- SppIP
- 175483-64-4
- Sakacin P inducer peptide
- Bacterial
- porcine reproductive and respiratory syndrome
- sakP
- porcine reproductive and respiratory syndrome virus glycoprotein 5
- porcine circovirus type 2d infection
- sakacin P structural gene
- Lactobacillus plantarum
- PCV2d capsid protein
- SARS-CoV-2 spike protein
- Lactobacillus sake LTH673
- lactic acid bacteria
- peptide
- Inhibitor
- inhibitor
- inhibit