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Light green SF yellowish  (Synonyms: Acid Green 5)

Cat. No.: HY-126367 Purity: 75.0%
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Light green SF yellowish (Acid Green 5) is a water-soluble triarylmethane dye. Light green SF yellowish serves as a histological stain that selectively labels mitochondria, collagen, and cartilage, while being an essential component of Papanicolaou staining. Light green SF yellowish is commonly used as a cytoplasmic counterstain for nuclear stains, and is applied in Masson's trichrome staining for collagen fibers, Pap staining, and cytological polychromatic staining in histopathology. Light green SF yellowish also induces growth inhibition and local fibrosarcomas in rats and exerts mild pulmonary tumorigenicity in mice.

For research use only. We do not sell to patients.

Light green SF yellowish

Light green SF yellowish Chemical Structure

CAS No. : 5141-20-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 g In-stock
10 g In-stock
50 g   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Light green SF yellowish Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Light green SF yellowish

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Light green SF yellowish (Acid Green 5) is a water-soluble triarylmethane dye. Light green SF yellowish serves as a histological stain that selectively labels mitochondria, collagen, and cartilage, while being an essential component of Papanicolaou staining. Light green SF yellowish is commonly used as a cytoplasmic counterstain for nuclear stains, and is applied in Masson's trichrome staining for collagen fibers, Pap staining, and cytological polychromatic staining in histopathology. Light green SF yellowish also induces growth inhibition and local fibrosarcomas in rats and exerts mild pulmonary tumorigenicity in mice[1][2][3][4].

In Vivo

Light green SF yellowish (30 mg; subcutaneous injection; once weekly; 2 years) causes a high incidence of injection-site fibrosarcomas[3].
Light green SF yellowish (1.0-2.0% dietary concentration; dietary; continuously; 2 years) increases survival at 1.0% dietary concentration, decreases survival at 2.0% dietary concentration, and produces a statistically significant increase in total lung tumour incidence without increasing malignant lung tumour or other organ tumour incidence when fed to C3HeB/FeJ mice for 2 years[3].
Light green SF yellowish (0.5-5.0% dietary concentrations; dietary; continuously; 2 years) induces growth inhibition in Osborne-Mendel rats at high dietary levels, with no significant differences in tumour incidence or reagent-related lesions[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Osborne-Mendel (weanling, 50 per group, evenly divided by sex)[3]
Dosage: 0.5%, 1.0%, 2.0%, 5.0% dietary concentrations
Administration: dietary; continuously; 2 years
Result: Induced growth inhibition in male rats at 2.0% and 5.0% dietary levels, and in female rats at 5.0% dietary level.
Showed no statistically significant differences in tumour incidence (including malignant tumours) between treated groups and controls.
Exhibited no gross or microscopic lesions attributable to the reagent.
Molecular Weight

792.85

Formula

C37H34N2Na2O9S3
CAS No.
Appearance

Solid

Color

Purple to purplish red

SMILES

O=S(C1=CC(CN(CC)C2=CC=C(/C(C3=CC=C(S(=O)(O[Na])=O)C=C3)=C4C=C/C(C=C/4)=[N+](CC)\CC5=CC=CC(S(=O)([O-])=O)=C5)C=C2)=CC=C1)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (63.06 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 5 mg/mL (6.31 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2613 mL 6.3064 mL 12.6127 mL
5 mM 0.2523 mL 1.2613 mL 2.5225 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.2613 mL 6.3064 mL 12.6127 mL 31.5318 mL
5 mM 0.2523 mL 1.2613 mL 2.5225 mL 6.3064 mL
DMSO 10 mM 0.1261 mL 0.6306 mL 1.2613 mL 3.1532 mL
15 mM 0.0841 mL 0.4204 mL 0.8408 mL 2.1021 mL
20 mM 0.0631 mL 0.3153 mL 0.6306 mL 1.5766 mL
25 mM 0.0505 mL 0.2523 mL 0.5045 mL 1.2613 mL
30 mM 0.0420 mL 0.2102 mL 0.4204 mL 1.0511 mL
40 mM 0.0315 mL 0.1577 mL 0.3153 mL 0.7883 mL
50 mM 0.0252 mL 0.1261 mL 0.2523 mL 0.6306 mL
60 mM 0.0210 mL 0.1051 mL 0.2102 mL 0.5255 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Light green SF yellowish Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Light green SF yellowish5141-20-8Acid Green 5Acid Green5Acid Green-5Fluorescent DyeC3HeB/FeJ miceOsborne-Mendel ratsdeoiled soyabeagle dogsfibrosarcomasmitochondriacartilagepulmonary tumorsbottom ashcollagenInhibitorinhibitorinhibit

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