2. Academic Validation
  3. Nanoengineered chlorin e6 conjugated with hydrogel for photodynamic therapy on cancer

Nanoengineered chlorin e6 conjugated with hydrogel for photodynamic therapy on cancer

  • Colloids Surf B Biointerfaces. 2019 Sep 1:181:778-788. doi: 10.1016/j.colsurfb.2019.06.040.
Shanmugapriya Karuppusamy 1,  Kim Hyejin 2,  Hyun Wook Kang 3
Affiliations

Affiliations

  • 1 Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology (BK 21 Plus), Pukyong National University, Busan, South Korea.
  • 2 Interdisciplinary program of Biomedical Mechanical & Electrical Engineering, Pukyong National University, Busan, South Korea.
  • 3 Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology (BK 21 Plus), Pukyong National University, Busan, South Korea; Interdisciplinary program of Biomedical Mechanical & Electrical Engineering, Pukyong National University, Busan, South Korea. Electronic address: [email protected].
PMID: 31238210 DOI: 10.1016/j.colsurfb.2019.06.040
Abstract

The aim of the present study is to fabricate hydrogel as a photosensitizer (PS) for photodynamic therapy. Chlorin e6 (Ce6)-fucoidan/alginates@gellam gum (Ce6-Fu/AL@GG)-based hydrogel was fabricated and characterised in terms of morphology and functional groups. MTT assay was used to check toxicity and also performed scratch assay for wound healing property of Ce6-Fu/AL@GGH. Fourier transform infrared spectroscopy (FT-IR) confirmed the existence of physical interactions between Polysaccharides. Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC) analysis confirmed a decrease in the thermal stability of the fabricated hydrogel. Scanning electron microscope (SEM) and Transmission electron microscope (TEM) images demonstrated porous matrixes representing homogeneous dispersion of nanoparticles in the hydrogel. Cytotoxicity tests revealed that a decrease in the cell viability occurred in PDT after 48 h treatment. Both Ce6 and laser irradiation induced the HT-29 apoptotic effect that was mediated by intracellular ROS generation and mitochondrial damage. The laser-treated hydrogel was effective in inhibiting HT-29 cell growth. Ce6-Fu/AL@GG hydrogel can be a promising platform for PDT on Cancer treatment.

Keywords

Anticancer; Chlorin e6; Hydrogel; Photodynamic therapy; Polysaccharides; ROS generation.

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