2. Apoptosis Epigenetics Cell Cycle/DNA Damage Others
  3. Bcl-2 Family Caspase PARP Apoptosis Fluorescent Dye
  4. Chlorin e6

Chlorin e6 is a photosensitizer and has strong absorption peaks at wavelength of 402 and 662 nm, as well as exhibiting intense fluorescence at 668 nm. Chlorin e6 has antimicrobial efficacy and anticancer activity. Chlorin e6 induces cell apoptosis via caspase-3 activation and can be used for the research of cancer.

For research use only. We do not sell to patients.

Chlorin e6 Chemical Structure

Chlorin e6 Chemical Structure

CAS No. : 19660-77-6

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Based on 5 publication(s) in Google Scholar

Chlorin e6 Related Antibodies

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PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Chlorin e6 is a photosensitizer and has strong absorption peaks at wavelength of 402 and 662 nm, as well as exhibiting intense fluorescence at 668 nm. Chlorin e6 has antimicrobial efficacy and anticancer activity. Chlorin e6 induces cell apoptosis via caspase-3 activation and can be used for the research of cancer[1][2][3].

IC50 & Target

Leishmania

 

Bax

 

Bcl-2

 

Caspase 3

 

PARP-1

 

In Vitro

Chlorin e6 (100 μg/mL, 24 h) with laser light (635 nm) exhibits no toxicity viability in HT-29 cells[2].
Chlorin e6 (5-10 μM, 3 h) with near-infrared laser light (660 nm) induces pancreatic cancer cell apoptosis via caspase-3 activation, resulting in tumor growth suppression in AsPC-1 and MIA PaCa-2 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chlorin e6 Related Antibodies

Western Blot Analysis[3]

Cell Line: AsPC-1 and MIA PaCa-2 Cells
Concentration: 5 μM, 10 μM
Incubation Time: 3 h
Result: Significantly reduced Bcl-2 protein expression while increasing apoptotic molecule expression of Bax.
Activated caspase-3 through its cleavage and upregulated the expression of cleaved PARP-1 in a dose-dependent manner.
In Vivo

Chlorin e6 (2.5 mg/kg, Intravenous injection, single dose) with a red light at a rate of 100 J/cm2 for 8 min 20 s has anti-tumor activity in B16F10 melanoma cells and PANC02 Pancreatic Cells xenograft mouse model[3].
Chlorin e6 (2.5 mg/kg, Intravenous injection, single dose) with near-infrared laser light (660 nm) suppresses the tumor growth in canine tumors[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: B16F10 melanoma cells xenograft mouse, PANC02 pancreatic cells xenograft mouse[3]
Dosage: 2.5 mg/kg
Administration: Intravenous injection (i.v.)
Result: Significantly reduced tumor volume.
Molecular Weight

596.67

Formula

C34H36N4O6
CAS No.
Appearance

Solid

Color

Light brown to black

SMILES

OC(C/C1=C2[C@H]([C@@H](C(/C=C3N/C(C(C=C)=C\3C)=C\C4=N/C(C(CC)=C4C)=C\C5=C(C)C(C(O)=O)=C1N5)=N\2)C)CCC(O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 20.83 mg/mL (34.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6760 mL 8.3798 mL 16.7597 mL
5 mM 0.3352 mL 1.6760 mL 3.3519 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.08 mg/mL (3.49 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 97.24%

COA (263 KB) HNMR (334 KB) RP-HPLC (250 KB) MS (85 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6760 mL 8.3798 mL 16.7597 mL 41.8992 mL
5 mM 0.3352 mL 1.6760 mL 3.3519 mL 8.3798 mL
10 mM 0.1676 mL 0.8380 mL 1.6760 mL 4.1899 mL
15 mM 0.1117 mL 0.5587 mL 1.1173 mL 2.7933 mL
20 mM 0.0838 mL 0.4190 mL 0.8380 mL 2.0950 mL
25 mM 0.0670 mL 0.3352 mL 0.6704 mL 1.6760 mL
30 mM 0.0559 mL 0.2793 mL 0.5587 mL 1.3966 mL
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Chlorin e6 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Chlorin e619660-77-6Ce6Chlorin e 6Chlorin e-6Ce 6Ce-6Bcl-2 FamilyCaspasePARPApoptosisFluorescent Dyepoly ADP ribose polymerase Mouse lewis lung cancer cell linesAsPC-1 cellsMIA PaCa-2 cellsB16F10 cellsB16F10 melanoma cells xenograft mouse ,PANC02 pancreatic cells xenograft mouseHT-29 cellsInhibitorinhibitorinhibit

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