2. Immunology/Inflammation
  3. Macrophage migration inhibitory factor (MIF)
  4. Mitomycin A

Mitomycin A is an antitumor agent. Mitomycin A inhibits the spontaneous migration of mouse monocytes. Mitomycin A inhibits the production of MIF (Migration Inhibition Factor) by human lymphocytes. Mitomycin A can be used in the research of tumor diseases.

For research use only. We do not sell to patients.

Mitomycin A

Mitomycin A Chemical Structure

CAS No. : 4055-39-4

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500 μg In-stock

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Based on 1 publication(s) in Google Scholar

Mitomycin A Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

Mitomycin A is an antitumor agent. Mitomycin A inhibits the spontaneous migration of mouse monocytes. Mitomycin A inhibits the production of MIF (Migration Inhibition Factor) by human lymphocytes. Mitomycin A can be used in the research of tumor diseases[1].

Cellular Effect
Cell Line Type Value Description References
A549 IC50
0.006 μg/mL
Compound: 16
in vitro anticancer activity against cultured A549 human tumor(lung) cell line
in vitro anticancer activity against cultured A549 human tumor(lung) cell line
[PMID: 11585450]
CCRF S-180 ED50
1.1 μg/mL
Compound: 28
Antitumor activity against mouse S180 cells implanted in ip dosed ddY mouse assessed as tumor volume after 7 days
Antitumor activity against mouse S180 cells implanted in ip dosed ddY mouse assessed as tumor volume after 7 days
[PMID: 19540628]
CCRF S-180 ED50
1.3 mg/kg
Compound: mitomycin A
Effective dose that gave 50 percent inhibition of sarcoma 180 tumor growth in ddY mice
Effective dose that gave 50 percent inhibition of sarcoma 180 tumor growth in ddY mice
[PMID: 8021919]
HCT-8 IC50
0.006 μg/mL
Compound: 16
in vitro inhibition of tumor cell growth in cultured HCT-8 human tumor cell line
in vitro inhibition of tumor cell growth in cultured HCT-8 human tumor cell line
[PMID: 11585450]
HeLa IC50
0.0024 μM
Compound: mitomycin A
In vitro anticellular activity tested against HeLa S3 cells
In vitro anticellular activity tested against HeLa S3 cells
[PMID: 8021918]
HeLa IC50
0.0024 μM
Compound: mitomycin A
In vitro anticellular activity against HeLa S3 cells
In vitro anticellular activity against HeLa S3 cells
[PMID: 8021919]
HepG2 IC50
0.008 μg/mL
Compound: 16
in vitro inhibition of tumor cell growth in cultured HepG2 human tumor cell line
in vitro inhibition of tumor cell growth in cultured HepG2 human tumor cell line
[PMID: 11585450]
KB IC50
0.008 μg/mL
Compound: 16
in vitro anticancer activity against cultured KB(epidermoid) human tumor cell line
in vitro anticancer activity against cultured KB(epidermoid) human tumor cell line
[PMID: 11585450]
L1210 IC50
0.0003 μM
Compound: 6
Inhibitory concentration on parentral (sensitive) L1210 leukemia cells.
Inhibitory concentration on parentral (sensitive) L1210 leukemia cells.
[PMID: 1906107]
L1210 IC50
0.0023 μM
Compound: 6
Inhibitory concentration on multidrug-resistant L1210 leukemia cells.
Inhibitory concentration on multidrug-resistant L1210 leukemia cells.
[PMID: 1906107]
MCF7 IC50
0.045 μg/mL
Compound: 16
in vitro inhibition of tumor cell growth in cultured MCF-7 human tumor cell line
in vitro inhibition of tumor cell growth in cultured MCF-7 human tumor cell line
[PMID: 11585450]
MCF7 IC50
0.63 nM
Compound: 20
Antitumor potency on human breast MCF-7 cell lines.
Antitumor potency on human breast MCF-7 cell lines.
[PMID: 1906109]
P388 IC50
0.24 nM
Compound: 20
Antitumor potency on P388 leukemia cells in culture
Antitumor potency on P388 leukemia cells in culture
[PMID: 1906109]
PC-3 IC50
0.02 μg/mL
Compound: 16
in vitro inhibition of tumor cell growth in cultured PC-3 human tumor cell line
in vitro inhibition of tumor cell growth in cultured PC-3 human tumor cell line
[PMID: 11585450]
U-87MG ATCC IC50
0.009 μg/mL
Compound: 16
in vitro anticancer activity against cultured U87MG (glioblastoma) human tumor cell line
in vitro anticancer activity against cultured U87MG (glioblastoma) human tumor cell line
[PMID: 11585450]
WiDr IC50
0.052 nM
Compound: 20
Antitumor potency on human colon WIDR cell lines.
Antitumor potency on human colon WIDR cell lines.
[PMID: 1906109]
In Vitro

Mitomycin A (0.05-5 μg/mL; 24 h) inhibits the spontaneous migration of WEHI-3 mouse monocytes in a concentration-dependent manner[1].
Mitomycin A (0.25 μg/mL; 65 h) significantly inhibits the production of migration inhibitory factor (MIF) by pokeweed mitogen-activated human lymphocytes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Mitomycin A Related Antibodies

Cell Migration Assay[1]

Cell Line: WEHI-3 murine myelomonocytic leukemia cells
Concentration: 0.05 μg/mL; 0.1 μg/mL; 0.5 μg/mL; 5 μg/mL
Incubation Time: 24 h
Result: Exhibited a migration index (MI) of 1.01 ± 0.05 at 0.05 μg/mL.
Showed a migration index (MI) of 1.03 ± 0.03 at 0.1 μg/mL.
Reduced the mean migration index (MI) to 0.65 ± 0.01 at 0.5 μg/mL, with a statistically significant decrease.
Lowered the mean migration index (MI) to 0.52 ± 0.02 at 5 μg/mL, with a statistically significant decrease.
Caused no statistically significant decreases in migration at 0.05 and 0.1 μg/mL.
Molecular Weight

349.34

Formula

C16H19N3O6
CAS No.
Appearance

Solid

Color

Purple to purplish red

SMILES

O=C1C(C)=C(OC)C(C2=C1N3[C@@]([C@](N4)([H])[C@]4([H])C3)(OC)[C@@H]2COC(N)=O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Purity & Documentation

Purity: 98.3%

SDS (393 KB)

COA (260 KB)

Handling Instructions (2659 KB)
References
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Mitomycin A Related Classifications

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  • Dilution Calculator

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Mitomycin A4055-39-4Macrophage migration inhibitory factor (MIF)pokeweed mitogen-activated human lymphocytesWEHI-3 murine monocytesmurine monocytesneoplastic diseasesMigration Inhibition Factorhuman lymphocytesInhibitorinhibitorinhibit

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Mitomycin A
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