2. MAPK/ERK Pathway
  3. MAPKAPK2 (MK2)
  4. MMI-0100

MMI-0100 is a cell-permeant peptide inhibitor of mitogen activated protein kinase activated protein kinase II (MK2). MMI-0100 reduces intimal hyperplasia ex vivo and in vivo. MMI-0100 suppresses IL-6 expression without effect on IL-8 expression. MMI-0100 suppresses fibrotic processes such as vein graft disease.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

MMI-0100 Chemical Structure

MMI-0100 Chemical Structure

CAS No. : 1039342-24-9

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10 mg USD 480 In-stock
25 mg USD 950 In-stock
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Based on 1 publication(s) in Google Scholar

MMI-0100 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

MMI-0100 is a cell-permeant peptide inhibitor of mitogen activated protein kinase activated protein kinase II (MK2). MMI-0100 reduces intimal hyperplasia ex vivo and in vivo. MMI-0100 suppresses IL-6 expression without effect on IL-8 expression. MMI-0100 suppresses fibrotic processes such as vein graft disease[1].

In Vitro

Naphthofluorescein (compound 19) (0-10 μM; 24 hours) suppresses the HIF-1 reporter activity in a concentration-dependent manner. [1].
MMI-0100 (0.25 and 0.5 mM; 24 hours) slightly increases cell proliferation in both cell types compared to control cells treated with 20 ng/ml TNF-α alone[1].
MMI-0100 (1 mM) treatment also increases both EC (11%) and SMC (7%) proliferation as compared to control, this response is not as robust as that induced by treatment with 0.5 mM MMI-0100[1].
MMI-0100 does not induce EC apoptosis at any dose[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MMI-0100 Related Antibodies

Cell Viability Assay[1]

Cell Line: human endothelial cell (EC) and smooth muscle cell (SMC)
Concentration: 0.25, 0.5, and 1 mM
Incubation Time: 24 hours
Result: Slightly increased cell proliferation in both cell types compared to control cells.
In Vivo

MMI-0100 (100 μM; 28 days) inhibits intimal hyperplasia in a mouse vein graft model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 12-week-old C57Bl/6 wild type mice (intimal hyperplasia)[1]
Dosage: 100 μM
Administration: vein graft, 28 days
Result: Diminished wall thickness at all postoperative time points in vein grafts treated with MMI-0100, with a ratio of 2.6-fold thicker at 4 weeks, compared to 4.7-fold thicker at 4 weeks in control grafts.
Clinical Trial
Molecular Weight

2283.64

Formula

C98H171N37O26
CAS No.
Appearance

Solid

Color

White to off-white

Sequence Shortening

YARAAARQARAKALARQLGVAA
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (43.79 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.4379 mL 2.1895 mL 4.3790 mL
5 mM 0.0876 mL 0.4379 mL 0.8758 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 14.29 mg/mL (6.26 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.4379 mL 2.1895 mL 4.3790 mL 10.9474 mL
5 mM 0.0876 mL 0.4379 mL 0.8758 mL 2.1895 mL
10 mM 0.0438 mL 0.2189 mL 0.4379 mL 1.0947 mL
15 mM 0.0292 mL 0.1460 mL 0.2919 mL 0.7298 mL
20 mM 0.0219 mL 0.1095 mL 0.2189 mL 0.5474 mL
25 mM 0.0175 mL 0.0876 mL 0.1752 mL 0.4379 mL
30 mM 0.0146 mL 0.0730 mL 0.1460 mL 0.3649 mL
40 mM 0.0109 mL 0.0547 mL 0.1095 mL 0.2737 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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MMI-0100 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MMI-01001039342-24-9MMI0100MMI 0100MAPKAPK2 (MK2)Mitogen-activated protein kinase activated protein kinase 2MAP kinase activated protein kinase 2MAPK activated protein kinase 2MAPKAP kinase 2MK2intimal hyperplasiaIL-6 expressionfibrotic processesvein graftMAPKAP kinase IIInhibitorinhibitorinhibit

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