N-Hydroxysuccinimide
Based on 9 publication(s) in Google Scholar
N-Hydroxysuccinimide (HOSu; 1-Hydroxy-2,5-pyrrolidinedione) is a covalent crosslinker commonly used in bioconjugation technology with a primary amine group. N-Hydroxysuccinimide reacts with amino groups (-NH2) to form a stable amide bond, which can modify amino-containing biomolecules. N-Hydroxysuccinimide can be used, for example, for protein labeling with fluorescent dyes and enzymes, surface activation of chromatography supports, microbeads, nanoparticles and microarray slides, and chemical synthesis of peptides. N-Hydroxysuccinimide has a wide range of applications in biomaterial synthesis (such as collagen, chitosan crosslinking), drug delivery systems (such as hydrogel preparation) and tissue engineering.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.98%
- CAS 番号: 6066-82-6
- 分子式: C4H5NO3
- 分子量:115.09
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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HY-D0178
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Dansyl chloride -
HY-B0099
Edaravone -
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N-Hydroxysulfosuccinimide sodium -
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MES -
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DBU -
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Heparin sodium salt -
HY-N0322
Cholesterol (from animal) -
HY-B0015
Paclitaxel -
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Dimethyl sulfoxide, meets analytical specification of Ch.P. -
HY-Y0775
1-Bromo-2,5-pyrrolidinedione
MedChemExpress（MCE）の使用を引用している文献 N-Hydroxysuccinimide
More- J Nanobiotechnology. 2025 Aug 26;23(1):588. [Abstract]
- Adv Healthc Mater. 2026 Feb 21:e05684. [Abstract]
- Adv Healthc Mater. 2025 Dec;14(31):e2500114. [Abstract]
- Int J Biol Macromol. 2026 Jul 17:376:153590.
- View. 2026 Mar 22.
- Int J Pharm X. 2025 Nov 17:10:100448. [Abstract]
- Ecotoxicol Environ Saf. 2025 Nov 15:307:119433. [Abstract]
- Biochem Pharmacol. 2025 Oct:240:117117. [Abstract]
- Arab J Chem. 2025 Nov 14.
生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | EC50 |
>50 μM
Compound: NHS
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Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability incubated for 30 mins by cell titer-glo assay
Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability incubated for 30 mins by cell titer-glo assay
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[PMID: 35859867] |
| HEK293 | IC50 |
>50 μM
Compound: NHS
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Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability incubated for 30 mins in presence of CRBN-tracer by nanoBRET assay
Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability incubated for 30 mins in presence of CRBN-tracer by nanoBRET assay
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[PMID: 35859867] |
| HEK293 | IC50 |
>50 μM
Compound: NHS
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Cytotoxicity against permeabilized HEK293 cells assessed as inhibition of cell viability incubated for 30 mins in presence of CRBN-tracer by nanoBRET assay
Cytotoxicity against permeabilized HEK293 cells assessed as inhibition of cell viability incubated for 30 mins in presence of CRBN-tracer by nanoBRET assay
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[PMID: 35859867] |
体外実験
In the cross-linking experiment of collagen solution, N-Hydroxysuccinimide-activated adipic acid was used to confirm the formation of intermolecular cross-linking by SDS-PAGE. Fluorescence spectroscopy showed that the polarity of the collagen microenvironment increased after cross-linking. Atomic force microscopy observed that collagen molecules randomly aggregated, which can improve the self-assembly ability of collagen[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 6066-82-6
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性状 Solid
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分子量 115.09
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分子式 C4H5NO3
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Color White to off-white
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SMILES
O=C(N1O)CCC1=O
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別名
HOSu; 1-Hydroxy-2,5-pyrrolidinedione
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (9)
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Journal Impact Factor
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Most Recent
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J Nanobiotechnology
Targeted neural stem cell-derived extracellular vesicles loaded with Sinomenine alleviate diabetic peripheral neuropathy via WNT5a/TRPV1 pathway modulation. [Abstract]2025 Aug 26;23(1):588. PMID: 40855286 -
Adv Healthc Mater
An Injectable Liposome-Hydrogel Composite for Targeted Delivery of a Non-Lactylated Peptide to Rebalance Bone Metabolism in Hyperlipidemia. [Abstract]2026 Feb 21:e05684. PMID: 41721628 -
Adv Healthc Mater
2025 Dec;14(31):e2500114. PMID: 40384169 -
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Int J Pharm X
A blood-brain barrier-penetrating nanoreactor for tumor microenvironment modulation, precise MR imaging and synergistic therapy of glioma. [Abstract]2025 Nov 17:10:100448. PMID: 41362442 -
Ecotoxicol Environ Saf
PPARγ-responsive luciferase reporter system for high-throughput screening of chemical toxins with potential pulmonary fibrosis effects. [Abstract]2025 Nov 15:307:119433. PMID: 41273832 -
Biochem Pharmacol
Herbacetin mitigates oxidative stress and ferroptosis to protect against doxorubicin-induced cardiotoxicity. [Abstract]2025 Oct:240:117117. PMID: 40619025 -
溶剤 & 溶解度
体外:
H2O : ≥ 100 mg/mL (868.89 mM)
DMSO : 100 mg/mL (868.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (276 KB)
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SDS (416 KB)
- English - EN (416 KB)
- Français - FR (416 KB)
- Deutsch - DE (416 KB)
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- Italian - IT (416 KB)
- Korean - KR (416 KB)
- Portuguese - PT (416 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Zhang M, et al. Interactions of collagen molecules in the presence of N-hydroxysuccinimide activated adipic acid (NHS-AA) as a crosslinking agent. Int J Biol Macromol. 2011 Nov 1;49(4):847-54. [Content Brief]
[2]. Ishikawa S, et al. N-Hydroxysuccinimide Bifunctionalized Triblock Cross-Linker Having Hydrolysis Property for a Biodegradable and Injectable Hydrogel System. ACS Biomater Sci Eng. 2019 Nov 11;5(11):5759-5769. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 8.6889 mL | 43.4443 mL | 86.8885 mL | 217.2213 mL |
| 5 mM | 1.7378 mL | 8.6889 mL | 17.3777 mL | 43.4443 mL | |
| 10 mM | 0.8689 mL | 4.3444 mL | 8.6889 mL | 21.7221 mL | |
| 15 mM | 0.5793 mL | 2.8963 mL | 5.7926 mL | 14.4814 mL | |
| 20 mM | 0.4344 mL | 2.1722 mL | 4.3444 mL | 10.8611 mL | |
| 25 mM | 0.3476 mL | 1.7378 mL | 3.4755 mL | 8.6889 mL | |
| 30 mM | 0.2896 mL | 1.4481 mL | 2.8963 mL | 7.2407 mL | |
| 40 mM | 0.2172 mL | 1.0861 mL | 2.1722 mL | 5.4305 mL | |
| 50 mM | 0.1738 mL | 0.8689 mL | 1.7378 mL | 4.3444 mL | |
| 60 mM | 0.1448 mL | 0.7241 mL | 1.4481 mL | 3.6204 mL | |
| 80 mM | 0.1086 mL | 0.5431 mL | 1.0861 mL | 2.7153 mL | |
| 100 mM | 0.0869 mL | 0.4344 mL | 0.8689 mL | 2.1722 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
N-Hydroxysuccinimide 関連分類
HY-Y0623 関連分類
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