N-Hydroxysuccinimide
Based on 8 publication(s) in Google Scholar
N-Hydroxysuccinimide (HOSu; 1-Hydroxy-2,5-pyrrolidinedione) is a covalent crosslinker commonly used in bioconjugation technology with a primary amine group. N-Hydroxysuccinimide reacts with amino groups (-NH2) to form a stable amide bond, which can modify amino-containing biomolecules. N-Hydroxysuccinimide can be used, for example, for protein labeling with fluorescent dyes and enzymes, surface activation of chromatography supports, microbeads, nanoparticles and microarray slides, and chemical synthesis of peptides. N-Hydroxysuccinimide has a wide range of applications in biomaterial synthesis (such as collagen, chitosan crosslinking), drug delivery systems (such as hydrogel preparation) and tissue engineering.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 6066-82-6
- Formula: C4H5NO3
- Molecular Weight:115.09
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) N-Hydroxysuccinimide
More- J Nanobiotechnology. 2025 Aug 26;23(1):588. [Abstract]
- Adv Healthc Mater. 2026 Feb 21:e05684. [Abstract]
- Adv Healthc Mater. 2025 May 19:e2500114. [Abstract]
- View. 2026 Mar 22.
- Int J Pharm X. 2025 Nov 17:10:100448. [Abstract]
- Ecotoxicol Environ Saf. 2025 Nov 15:307:119433. [Abstract]
- Biochem Pharmacol. 2025 Oct:240:117117. [Abstract]
- Arab J Chem. 2025 Nov 14.
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | EC50 |
>50 μM
Compound: NHS
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Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability incubated for 30 mins by cell titer-glo assay
Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability incubated for 30 mins by cell titer-glo assay
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[PMID: 35859867] |
| HEK293 | IC50 |
>50 μM
Compound: NHS
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Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability incubated for 30 mins in presence of CRBN-tracer by nanoBRET assay
Cytotoxicity against human HEK293 cells assessed as inhibition of cell viability incubated for 30 mins in presence of CRBN-tracer by nanoBRET assay
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[PMID: 35859867] |
| HEK293 | IC50 |
>50 μM
Compound: NHS
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Cytotoxicity against permeabilized HEK293 cells assessed as inhibition of cell viability incubated for 30 mins in presence of CRBN-tracer by nanoBRET assay
Cytotoxicity against permeabilized HEK293 cells assessed as inhibition of cell viability incubated for 30 mins in presence of CRBN-tracer by nanoBRET assay
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[PMID: 35859867] |
In the cross-linking experiment of collagen solution, N-Hydroxysuccinimide-activated adipic acid was used to confirm the formation of intermolecular cross-linking by SDS-PAGE. Fluorescence spectroscopy showed that the polarity of the collagen microenvironment increased after cross-linking. Atomic force microscopy observed that collagen molecules randomly aggregated, which can improve the self-assembly ability of collagen[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 6066-82-6
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Appearance Solid
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Molecular Weight 115.09
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Formula C4H5NO3
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Color White to off-white
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SMILES
O=C(N1O)CCC1=O
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Synonyms
HOSu; 1-Hydroxy-2,5-pyrrolidinedione
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (8)
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Journal Impact Factor
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Most Recent
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J Nanobiotechnology
Targeted neural stem cell-derived extracellular vesicles loaded with Sinomenine alleviate diabetic peripheral neuropathy via WNT5a/TRPV1 pathway modulation. [Abstract]2025 Aug 26;23(1):588. PMID: 40855286 -
Adv Healthc Mater
An Injectable Liposome-Hydrogel Composite for Targeted Delivery of a Non-Lactylated Peptide to Rebalance Bone Metabolism in Hyperlipidemia. [Abstract]2026 Feb 21:e05684. PMID: 41721628 -
Adv Healthc Mater
2025 May 19:e2500114. PMID: 40384169 -
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Int J Pharm X
A blood-brain barrier-penetrating nanoreactor for tumor microenvironment modulation, precise MR imaging and synergistic therapy of glioma. [Abstract]2025 Nov 17:10:100448. PMID: 41362442 -
Ecotoxicol Environ Saf
PPARγ-responsive luciferase reporter system for high-throughput screening of chemical toxins with potential pulmonary fibrosis effects. [Abstract]2025 Nov 15:307:119433. PMID: 41273832 -
Biochem Pharmacol
Herbacetin mitigates oxidative stress and ferroptosis to protect against doxorubicin-induced cardiotoxicity. [Abstract]2025 Oct:240:117117. PMID: 40619025 -
Solvent & Solubility
H2O : ≥ 100 mg/mL (868.89 mM)
DMSO : 100 mg/mL (868.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (416 KB)
- English - EN (416 KB)
- Français - FR (416 KB)
- Deutsch - DE (416 KB)
- Norwegian - NO (416 KB)
- Español - ES (416 KB)
- Swedish - SV (416 KB)
- Italian - IT (416 KB)
- Korean - KR (416 KB)
- Portuguese - PT (416 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhang M, et al. Interactions of collagen molecules in the presence of N-hydroxysuccinimide activated adipic acid (NHS-AA) as a crosslinking agent. Int J Biol Macromol. 2011 Nov 1;49(4):847-54. [Content Brief]
[2]. Ishikawa S, et al. N-Hydroxysuccinimide Bifunctionalized Triblock Cross-Linker Having Hydrolysis Property for a Biodegradable and Injectable Hydrogel System. ACS Biomater Sci Eng. 2019 Nov 11;5(11):5759-5769. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 8.6889 mL | 43.4443 mL | 86.8885 mL | 217.2213 mL |
| 5 mM | 1.7378 mL | 8.6889 mL | 17.3777 mL | 43.4443 mL | |
| 10 mM | 0.8689 mL | 4.3444 mL | 8.6889 mL | 21.7221 mL | |
| 15 mM | 0.5793 mL | 2.8963 mL | 5.7926 mL | 14.4814 mL | |
| 20 mM | 0.4344 mL | 2.1722 mL | 4.3444 mL | 10.8611 mL | |
| 25 mM | 0.3476 mL | 1.7378 mL | 3.4755 mL | 8.6889 mL | |
| 30 mM | 0.2896 mL | 1.4481 mL | 2.8963 mL | 7.2407 mL | |
| 40 mM | 0.2172 mL | 1.0861 mL | 2.1722 mL | 5.4305 mL | |
| 50 mM | 0.1738 mL | 0.8689 mL | 1.7378 mL | 4.3444 mL | |
| 60 mM | 0.1448 mL | 0.7241 mL | 1.4481 mL | 3.6204 mL | |
| 80 mM | 0.1086 mL | 0.5431 mL | 1.0861 mL | 2.7153 mL | |
| 100 mM | 0.0869 mL | 0.4344 mL | 0.8689 mL | 2.1722 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
N-Hydroxysuccinimide Related Classifications
HY-Y0623 Related Classifications
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