N3-Cho bromide
Based on 1 Customer Validation
N3-Cho (Azido-choline) bromide is a click chemistry reagent containing an azide group that can be used in the synthesis of cell membrane structures.
For research use only. We do not sell to patients.
- Purity : 99.63%
- CAS No.: 2059973-54-3
- Formula: C6H15BrN4O
- Molecular Weight:239.11
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Click chemistry is used to describe the selective, modular, wide range and high-yield chemical reactions that allow rapid synthesis of new compounds through heteroatom linking (C-X-C). Bertozzi developed click chemistry in a new dimension —Bioorthogonal chemistry. It is defined as a rapid and selective reaction that does not interfere with biological processes under physiological conditions. Common reaction structures of click chemistry, such as Azide, Alkyne, DBCO, BCN, TCO and Tetrazine.?
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2059973-54-3
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Appearance Solid-Liquid Mixture
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Molecular Weight 239.11
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Formula C6H15BrN4O
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Color Colorless to off-white
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SMILES
[N-]=[N+]=NCC[N+](C)(CCO)C.[Br-]
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Synonyms
Azido-choline bromide
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
H2O : 125 mg/mL (522.77 mM; Need ultrasonic)
DMSO : 100 mg/mL (418.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 4.1822 mL | 20.9109 mL | 41.8218 mL | 104.5544 mL |
| 5 mM | 0.8364 mL | 4.1822 mL | 8.3644 mL | 20.9109 mL | |
| 10 mM | 0.4182 mL | 2.0911 mL | 4.1822 mL | 10.4554 mL | |
| 15 mM | 0.2788 mL | 1.3941 mL | 2.7881 mL | 6.9703 mL | |
| 20 mM | 0.2091 mL | 1.0455 mL | 2.0911 mL | 5.2277 mL | |
| 25 mM | 0.1673 mL | 0.8364 mL | 1.6729 mL | 4.1822 mL | |
| 30 mM | 0.1394 mL | 0.6970 mL | 1.3941 mL | 3.4851 mL | |
| 40 mM | 0.1046 mL | 0.5228 mL | 1.0455 mL | 2.6139 mL | |
| 50 mM | 0.0836 mL | 0.4182 mL | 0.8364 mL | 2.0911 mL | |
| 60 mM | 0.0697 mL | 0.3485 mL | 0.6970 mL | 1.7426 mL | |
| 80 mM | 0.0523 mL | 0.2614 mL | 0.5228 mL | 1.3069 mL | |
| 100 mM | 0.0418 mL | 0.2091 mL | 0.4182 mL | 1.0455 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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