Neuroprotectin B
Neuroprotectin B is a cyclopeptide. Neuroprotectin B has the function of protecting chicken brain neuron culture from excitatory toxicity induced by glutamate and red alginate.
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- CAS. Nr.: 354986-38-2
- Formel: C61H45Cl6N7O17
- Molecular Weight:1360.76
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Biologische Aktivität
Beschreibung
Chemical Information
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CAS. Nr. 354986-38-2
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Molecular Weight 1360.76
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Formel C61H45Cl6N7O17
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SMILES
OC(C(OC1=CC=C(C[C@H](N2C)C(N[C@H](C3=CC=C(O)C=C3)C(O)=O)=O)C=C1)=CC([C@@H](N4)C(N[C@H](C5=CC(Cl)=C(O)C(Cl)=C5)C2=O)=O)=C6)=C6C(C=C7)=CC(N8)=C7C(C[C@@H](NC(C(C9=CC(Cl)=C(O)C(Cl)=C9)=O)=O)C(N[C@H](C%10=CC(Cl)=C(O)C(Cl)=C%10)C4=O)=O)(O)C8=O
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Structure Classification
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Initial Source
Streptomyces sp. MA7234
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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iPSC/hPSC-Derived Neuron Differentiation Culture
iPSC/hPSC-derived neuron differentiation culture directs pluripotent cells toward neuroectoderm and then neuronal lineages by suppressing developmental signals that maintain non-neural fates; the classic monolayer dual-SMAD approach blocks BMP and Activin/TGF-β signaling with Noggin or dorsomorphin/LDN193189 plus SB431542, producing PAX6-positive neural progenitors that can be further matured into neurons. The readout is generated by morphology, neural progenitor markers, neuronal markers, subtype markers, and functional assays: PAX6/SOX1/NESTIN indicate neural progenitor induction, βIII-tubulin/TUJ1 and MAP2 indicate neuronal differentiation, cortical programs can be assessed by FOXG1, TBR1, CTIP2, SATB2, and synaptic maturation can be assessed by synaptic proteins, calcium activity, multielectrode arrays, or patch-clamp electrophysiology.
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Serum-Free B27/Neurobasal Neuronal Maintenance Culture
Serum-free B27/Neurobasal culture is a defined neuronal maintenance method designed to support dissociated primary neurons while limiting serum-driven glial expansion; the readout is sustained neuronal survival, neurite extension, neuronal marker expression, synapse formation, and, when measured, electrophysiological or calcium activity. B27/Neurobasal was optimized in embryonic rat hippocampal neurons, where B27 supported >60% survival after 4 days above 160 plated cells/mm2 and Neurobasal reduced glial growth to <0. 5% by immunocytochemistry; later studies extended the approach to cortex, striatum, substantia nigra, septum, cerebellum, and dentate gyrus neurons.
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Directly Induced Neuron Culture
Directly induced neuron culture converts somatic cells, most commonly fibroblasts, into induced neurons without passing through a pluripotent or neural progenitor stage; classic evidence shows that mouse fibroblasts can be converted by Ascl1, Brn2/Pou3f2, and Myt1l, human fibroblasts can be converted by defined neuronal transcription factors, and human fibroblasts can also be converted by miR-9/9-124 with neurogenic or subtype-specifying transcription factors. The readout is acquisition of neuronal identity and function, assessed by neuronal morphology, neuronal markers such as Tuj1/βIII-tubulin, MAP2, synapsin, and subtype markers when relevant, together with functional assays such as action-potential firing, synaptic activity, and electrophysiology.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Neuroprotectin B Related Classifications
HY-N14868 Related Classifications
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