Cat. No.: HY-D1373

HBC

(Synonyms: HBC 530)

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

HBC (HBC 530) is a GFP fluorophore-like synthetic dye, with a structurally rigid electron acceptor and a strong electron donor. HBC has a low fluorescence background, and when combined with Pepper (RNA aptamer), HBC forms a tight complex and activates and emits bright fluorescence (K_d of ~3.5 nM). HBC emission peaks vary in different complexes and covers the spectrum from cyan to red. HBC can be used in the live cell imaging of RNA (Em/Ex = 530/485 nm).

For research use only. We do not sell to patients.

Purity: 99.36%
CAS No.: 156840-13-0
Formula: C₁₉H₁₇N₃O
Molecular Weight:303.36

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

HBC Chemical Structure

CAS No.: 156840-13-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- Clinical Trial
- Emission (Em)
- Excitation (Ex)
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

Native polyacrylamide gel electrophoresis (PAGE) analysis
Native PAGE conditions included 8% PAGE gels (29:1 acrylamide:bisacrylamide) using TBE buffer.
The samples are run at 40 V for at least 2 h in pre-cooled buffer in an ice bath.
For staining, gels are incubated in buffer (40 mM HEPES, pH 7.5, 100 mM KCl, 5 mM MgCl2) containing 10 μM HBC for 15 min.
Gels are then imaged to detect Pepper-HBC complexes. The stained gels are analyzed at 488/30 nm excitation and 535/30 nm emission.
Next, counterstaining with 1× SYBR Gold is performed for 15 min followed by gel imaging to detect all RNA species in each lane.
SYBR Gold-stained bands are imaged using ultraviolet excitation (302 nm) and 590/30 nm emission.

Mammalian cell flow cytometry
293T/17 cells are transfected with the corresponding plasmids. Empty plasmid without expression of aptamer is used as the control. Untransfected cells are used as a negative control.
After 30 h, the cells are digested and resuspended in the 4% FBS/1× PBS solution containing 0.2 μM HBC and 5 mM MgSO4.
The fluorescence is analyzed using a cytometer with two channels: Ex=488/8 nm, Em=525/40 nm and Ex=561/10 nm, Em=610/20 nm.

To study the correlation between mRNA and protein, cells are transfected with plasmids expressing different mRNAs tagged with 4Pepper and cultured for 24 h.
The cells are digested and resuspended in the 4% FBS/1× PBS solution containing 0.2 μM HBC and 5 mM MgSO4.
Cells expressing SNAP-4Pepper are pre-treated with 0.2 μM SNAP-Cell 647-SiR (NEB).
Ex=405/10 nm, Em=450/45 nm, Ex=488/8 nm, Em=525/40 nm, Ex=561/10 nm, Em=610/20 nm and Ex=638 nm, Em=660/20 nm channels are used for blue fluorescent protein, Pepper530, mCherry and SNAP-SiR, respectively.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Emission (Em)

530

Excitation (Ex)

485

Chemical Information

CAS No. 156840-13-0
Appearance Solid
Molecular Weight 303.36
Formula C₁₉H₁₇N₃O
Color Orange to red
SMILES

N#CC1=CC=C(/C(C#N)=C\C2=CC=C(N(CCO)C)C=C2)C=C1
Synonyms

HBC 530
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (3)

All Bio/Physico-chemical AssayCell Imaging/Staining

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Nature

De novo design of transmembrane fluorescence-activating proteins. [Abstract]

2025 Apr;640(8057):249-257. PMID: 39972138

HBC purchased from MedChemExpress. Usage Cited in: Nature. 2025 Apr;640(8057):249-257. [Abstract]

Fluorescence activation of three HBC derivatives (HBC530, HBC599 and HBC620) by different wFAPs. Solutions containing the HBC dyes, with or without wFAPs, were illuminated with 365 nm light.

HBC purchased from MedChemExpress. Usage Cited in: Nature. 2025 Apr;640(8057):249-257. [Abstract]

Activation of the fluorescence of HBC dyes (HBC530, HBC599 and HBC620) by tmFAP1.1 and tmFAP1.2. Detergent solutions containing HBC dyes, with or without tmFAPs, were illuminated with 365 nm light.All tmFAPs were solubilized in TBS buffer (pH 7.4) with 1.1% n-octyl-β-d-glucopyranoside.
Aggregate

2026 Feb 4;7(2):e70284.

HBC purchased from MedChemExpress. Usage Cited in: Aggregate. 2026 Feb 4;7(2):e70284.

Excitation and emission spectra of ThT and HBC530 using Pepper.

HBC purchased from MedChemExpress. Usage Cited in: Aggregate. 2026 Feb 4;7(2):e70284.

Confocal fluorescence imaging of F-FARCONwith and without 2.5% PEG 8K. Fluorescence images were taken in solutions containing 2 µM RNA, 1 mM MgCl 2 and 20 µM HBC530 after annealing.
Anal Chim Acta

The catalytic hairpin assembly induces a conformational change of the RNA aptamer enabled in situ imaging of circRNAs in tumor cells. [Abstract]

2025 Jun 22:1356:344048. PMID: 40288881

HBC purchased from MedChemExpress. Usage Cited in: Anal Chim Acta. 2025 Jun 22:1356:344048. [Abstract]

In situ imaging of circMTO1 in LO2 cells with and without inert pepper aptamer (i-pepper aptamer). The cells were transfected with H1, H2, inert Pepper aptamer, and RiboLock RNase Inhibitor for 6 h with transfection reagent, and then incubated with HBC530 for 4 h. The magnification of the confocal microscope was 20 ×.

HBC purchased from MedChemExpress. Usage Cited in: Anal Chim Acta. 2025 Jun 22:1356:344048. [Abstract]

In situ imaging of circMTO1 in HeLa cells, LO2 cells, HepG2 cells.

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (412.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.2964 mL

16.4821 mL

32.9641 mL

5 mM

0.6593 mL

3.2964 mL

6.5928 mL

10 mM

0.3296 mL

1.6482 mL

3.2964 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chen X, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol. 2019;37(11):1287-1293. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.2964 mL	16.4821 mL	32.9641 mL	82.4103 mL
	5 mM	0.6593 mL	3.2964 mL	6.5928 mL	16.4821 mL
	10 mM	0.3296 mL	1.6482 mL	3.2964 mL	8.2410 mL
	15 mM	0.2198 mL	1.0988 mL	2.1976 mL	5.4940 mL
	20 mM	0.1648 mL	0.8241 mL	1.6482 mL	4.1205 mL
	25 mM	0.1319 mL	0.6593 mL	1.3186 mL	3.2964 mL
	30 mM	0.1099 mL	0.5494 mL	1.0988 mL	2.7470 mL
	40 mM	0.0824 mL	0.4121 mL	0.8241 mL	2.0603 mL
	50 mM	0.0659 mL	0.3296 mL	0.6593 mL	1.6482 mL
	60 mM	0.0549 mL	0.2747 mL	0.5494 mL	1.3735 mL
	80 mM	0.0412 mL	0.2060 mL	0.4121 mL	1.0301 mL
	100 mM	0.0330 mL	0.1648 mL	0.3296 mL	0.8241 mL

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HBC

Publications Citing Use of MedChemExpress (MCE) HBC

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

HBC Related Classifications

Cat. No.: HY-D1373