(S)-DMP2

(S)-DMP2 selectively enriches eIF4E in HEK293T cells^[1].
(S)-DMP2 (5-100 μM) labels eIF4E in HEK293T cells in a dose-dependent manner. This labeling disrupts the epitope recognized by mouse anti-eIF4E antibody, but does not affect the epitope recognized by rabbit anti-eIF4E antibody^[1].
(S)-DMP2 specifically binds to the cap-binding pocket of eIF4E and labels recombinant eIF4E in vitro, and this labeling process can be competitively blocked by m7GTPG and Taunton 12; in cellular assays, pretreatment with Taunton 12 inhibits the labeling of (S)-DMP2 and restores the binding of eIF4E antibody^[1].
(S)-DMP2 tag impairs the cap-binding activity of eIF4E in HEK293T cells, as evidenced by the reduced binding of eIF4E to m7GTP-modified agarose microspheres^[1].
(S)-DMP2 covalently modifies the Met101 and Glu103 residues within the cap-binding pocket of recombinantly expressed eIF4E^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.