2. Immunology/Inflammation Apoptosis
  3. NOD-like Receptor (NLR) AIM2 Pyroptosis
  4. NLRP3/AIM2-IN-3

NLRP3/AIM2-IN-3 (compound 59) is a potent inhibitor with differential species specific effects against NLRP3 and AIM2 inflammasome-dependent pyroptosis. NLRP3/AIM2-IN-3 shows inhibitory efficacy against pyroptosis in THP-1 macrophages stimulated with LPS/nigericin, with an IC50 of 0.077 ± 0.008 μM. NLRP3/AIM2-IN-3 disturbs the interaction of NLRP3 or AIM2 with the adaptor protein ASC and inhibited ASC oligomerization.

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CAS No. : 1787787-60-3

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Customer Review

Based on 10 publication(s) in Google Scholar

NLRP3/AIM2-IN-3 Related Antibodies

Top Publications Citing Use of Products

    NLRP3/AIM2-IN-3 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Jan 24;44(2):115177.  [Abstract]

    NLRP3/AIM2-IN-3 (AIM2/NLRP3 inhibitor) (10 μM; 30 min) treatment on BMDCs led to earlier phosphorylation of TBK1, STAT1, and STING from microsatellite-containing oligos.

    NLRP3/AIM2-IN-3 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2025 Dec 29;83(1):48.  [Abstract]

    NLRP3/AIM2-IN-3 (10 µM; 1 h) notably enhanced viability of MLE-12 cells stimulated with LPS.

    NLRP3/AIM2-IN-3 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2025 Dec 29;83(1):48.  [Abstract]

    NLRP3/AIM2-IN-3 (10 µM; 1 h) effectively reversed the increased proportions of PI-positive and Caspase-1-positive cells in LPS-stimulated MLE-12 cells.

    NLRP3/AIM2-IN-3 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2025 Dec 29;83(1):48.  [Abstract]

    NLRP3/AIM2-IN-3 (10 µM; 1 h) significantly reduced IL-1β and IL-18 concentrations in the cell culture supernatant of LPS-stimulated MLE-12 cells.

    NLRP3/AIM2-IN-3 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2025 Dec 29;83(1):48.  [Abstract]

    NLRP3/AIM2-IN-3 (10 µM; 1 h) significantly reduced the expression levels of the pyroptosis-related protein GSDMD-N in LPS-stimulated MLE-12 cells.

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    NLRP3 NLRP1 NOD1 NOD2

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    Description

    NLRP3/AIM2-IN-3 (compound 59) is a potent inhibitor with differential species specific effects against NLRP3 and AIM2 inflammasome-dependent pyroptosis. NLRP3/AIM2-IN-3 shows inhibitory efficacy against pyroptosis in THP-1 macrophages stimulated with LPS/nigericin, with an IC50 of 0.077 ± 0.008 μM. NLRP3/AIM2-IN-3 disturbs the interaction of NLRP3 or AIM2 with the adaptor protein ASC and inhibited ASC oligomerization[1].

    IC50 & Target

    NLRP3

     

    In Vitro

    NLRP3/AIM2-IN-3 (0-100 μM) displays remarkable inhibitory activity against NLRP3-and AIM2-but not NLRC4-dependent activation of caspase-1 and the release of IL-1b in human THP-1 macrophages[1].
    NLRP3/AIM2-IN-3 shows inhibition of IL-1b secretion by human THP-1 macrophages, with IC50 of 0.098 μM, which is nearly 150-fold and 500-fold more potent than that of J774A.1 (14.62 μM) and bone marrow-derived macrophages (BMDMs) (48.98 μM), respectively[1].
    NLRP3/AIM2-IN-3 has almost no inhibitory effect on the AIM2 inflammasome-dependent activation of caspase-1 and release of IL-1b in J774A.1 cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    NLRP3/AIM2-IN-3 Related Antibodies
    Molecular Weight

    266.29

    Formula

    C16H14N2O2
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    O=C(NC1=CC=CC(O)=C1)CC2=CC3=C(C=C2)C=CN3
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 250 mg/mL (938.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Methanol : 50 mg/mL (187.77 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7553 mL 18.7765 mL 37.5530 mL
    5 mM 0.7511 mL 3.7553 mL 7.5106 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Volume
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (7.81 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.23%

    SDS (251 KB)

    COA (245 KB) HNMR (253 KB) LCMS (87 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Methanol / DMSO 1 mM 3.7553 mL 18.7765 mL 37.5530 mL 93.8826 mL
    5 mM 0.7511 mL 3.7553 mL 7.5106 mL 18.7765 mL
    10 mM 0.3755 mL 1.8777 mL 3.7553 mL 9.3883 mL
    15 mM 0.2504 mL 1.2518 mL 2.5035 mL 6.2588 mL
    20 mM 0.1878 mL 0.9388 mL 1.8777 mL 4.6941 mL
    25 mM 0.1502 mL 0.7511 mL 1.5021 mL 3.7553 mL
    30 mM 0.1252 mL 0.6259 mL 1.2518 mL 3.1294 mL
    40 mM 0.0939 mL 0.4694 mL 0.9388 mL 2.3471 mL
    50 mM 0.0751 mL 0.3755 mL 0.7511 mL 1.8777 mL
    60 mM 0.0626 mL 0.3129 mL 0.6259 mL 1.5647 mL
    80 mM 0.0469 mL 0.2347 mL 0.4694 mL 1.1735 mL
    100 mM 0.0376 mL 0.1878 mL 0.3755 mL 0.9388 mL
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    NLRP3/AIM2-IN-3 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    NLRP3/AIM2-IN-31787787-60-3NOD-like Receptor (NLR)AIM2PyroptosisAbsent in melanoma 2Interferon-inducible protein AIM2PYHINNLRP3 inflammasomeAIM2 inflammasomePyroptosis inhibitorSpecies differencesASC oligomerizationInhibitorinhibitorinhibit

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