NSC 1008
NSC 1008 is a selective Ras converting enzyme 1 (Rce1) inhibitor that reduces Rce1 in vitro activity with a human IC50 of 8.9 μM. NSC 1008 demonstrates selectivity for Rce1 by not inhibiting human Ste24 or huamn FTase. NSC 1008 exhibits low cell toxicity, and induces mislocalization of EGFP-Ras from the plasma membrane in cancer cells. NSC 1008 can be used for the research of cancer.
For research use only. We do not sell to patients.
- CAS No.: 5335-95-5
- Formula: C22H18N2O
- Molecular Weight:326.40
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| U-87MG ATCC | IC50 |
>10 μM
Compound: NC266, NSC 1008
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Cytotoxicity against human U-87 MG cells assessed as growth inhibition measured after 72 hrs by MTT assay
Cytotoxicity against human U-87 MG cells assessed as growth inhibition measured after 72 hrs by MTT assay
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[PMID: 32830969] |
| U-87MG ATCC | IC50 |
10 μM
Compound: NC266, NSC 1008
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Cytotoxicity against human U-87 MG cells assessed as growth inhibition measured after 72 hrs by MTT assay
Cytotoxicity against human U-87 MG cells assessed as growth inhibition measured after 72 hrs by MTT assay
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[PMID: 32830969] |
In Vitro
NSC 1008 (compound 2) inhibits human Rce1 activity (IC50 = 8.9 μM)[1].
NSC 1008 (25 µM; 20 hours) exhibits low toxicity in HCT-116 cells[1].
NSC 1008 (25 µM; 20 hours) induces mislocalization of EGFP-H-Ras, EGFP-N-Ras, and EGFP-K-Ras4b in HCT-116 cells[1].
NSC 1008 (10-50 µM) demonstrates selectivity for Rce1 by not inhibiting human Ste24 or huamn FTase[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HCT-116 human colon carcinoma cells
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Concentration:25 μM
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Incubation Time:20-hour treatment; 4-hour reagent incubation
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Result:Caused minimal cell death (less than 10%) compared to DMSO-treated and untreated cells.
Chemical Information
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CAS No. 5335-95-5
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Appearance Solid
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Molecular Weight 326.40
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Formula C22H18N2O
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Color White to off-white
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SMILES
OC=1C2=NC=CC=C2C=CC1C(NC=3C=CC=CC3)C=4C=CC=CC4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
NSC 1008 Related Classifications
HY-124126 Related Classifications
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