TES
Based on 1 Customer Validation
TES is an excellent hydrogen ion buffer. TES has characteristics such as an appropriate dissociation constant, a low metal-binding constant, and high water solubility. TES can be used in the research of mammalian cell culture, viruses, and so on.
For research use only. We do not sell to patients.
- Purity: 99.91%
- CAS No.: 7365-44-8
- Formula: C6H15NO6S
- Molecular Weight:229.25
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
TES (0.1 M) has pKa values of 7.92, 7.50, and 7.14 at 0°, 20° and 37°, respectively, and the concentration of its saturated aqueous solution at 0° is 2.6 M[1].
TES (6-50 mM; 11-14 days) in a closed culture environment is similar to or even more effective than the NaHCO3-CO2-buffered medium in terms of colony-forming efficiency, colony size, and cell morphology of several mammalian cell lines (HeLa S3, KB, BHK 21-Cl3, 3Y1-B Cl-1)[2].
TES (25-50 mM; 14-19 days) has a similar efficiency to NaHCO3-CO2-buffered medium and the required time is shorter in the SV40 virus plaque assay with monkey kidney CV-1 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 7365-44-8
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Appearance Solid
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Molecular Weight 229.25
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Formula C6H15NO6S
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Color White to off-white
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SMILES
OCC(NCCS(=O)(O)=O)(CO)CO
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
H2O : 125 mg/mL (545.26 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (436.21 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (272 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. N E Good, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467-77. [Content Brief]
[2]. A Itagaki, et al. Tes and HEPES buffers in mammalian cell cultures and viral studies: problem of carbon dioxide requirement. Exp Cell Res. 1974 Feb;83(2):351-61. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.3621 mL | 21.8103 mL | 43.6205 mL | 109.0513 mL |
| 5 mM | 0.8724 mL | 4.3621 mL | 8.7241 mL | 21.8103 mL | |
| 10 mM | 0.4362 mL | 2.1810 mL | 4.3621 mL | 10.9051 mL | |
| 15 mM | 0.2908 mL | 1.4540 mL | 2.9080 mL | 7.2701 mL | |
| 20 mM | 0.2181 mL | 1.0905 mL | 2.1810 mL | 5.4526 mL | |
| 25 mM | 0.1745 mL | 0.8724 mL | 1.7448 mL | 4.3621 mL | |
| 30 mM | 0.1454 mL | 0.7270 mL | 1.4540 mL | 3.6350 mL | |
| 40 mM | 0.1091 mL | 0.5453 mL | 1.0905 mL | 2.7263 mL | |
| 50 mM | 0.0872 mL | 0.4362 mL | 0.8724 mL | 2.1810 mL | |
| 60 mM | 0.0727 mL | 0.3635 mL | 0.7270 mL | 1.8175 mL | |
| 80 mM | 0.0545 mL | 0.2726 mL | 0.5453 mL | 1.3631 mL | |
| 100 mM | 0.0436 mL | 0.2181 mL | 0.4362 mL | 1.0905 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
TES Related Classifications
HY-23430 Related Classifications
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