Fluorescent Lipids

Laurdan is a membrane-permeable fluorescent probe that displays spectral sensitivity to the phospholipid phase of the cell membrane to which it is bound. Quantitation of generalized polarization (GP) of Laurdan can be used to identify phospholipid phase (Ex/Em = 370/440-490 nm).

Fluoresterol (22-NBD Cholesterol) is a cholesterol-specific fluorescent probe with cholesterol-mimicking binding properties. Fluoresterol is ineffective orally and does not cross the blood-brain barrier. Fluoresterol specifically binds to cholesterol transport-related proteins (such as ABCA1 and ABCG1) and is primarily used in cholesterol metabolism research, particularly for the visualization and quantitative analysis of cholesterol absorption, efflux, intracellular transport efficiency, and reverse cholesterol transport (RCT) processes. The commonly used concentration of Fluoresterol in in vitro experiments is 0.1-10 μM, and the commonly used dose in in vivo experiments is 5-20 mg/kg (gavage or intraperitoneal injection), with excitation/emission wavelengths of 472/540 nm. Fluoresterol can be applied to the study of cholesterol metabolism mechanisms related to hyperlipidemia, atherosclerosis, and non-alcoholic fatty liver disease (NAFLD).

18:1 Liss Rhod (18:1 Lissamine rhodamine) PE is a fluorescent phospholipid and fluorescent probe.18:1 Liss Rhod PE admixes into phospholipid inks for large-scale monitoring of dip-pen nanolithography-generated lithographic structures via fluorescence microscopy.18:1 Liss Rhod PE undergoes phase separation or self-quenching under certain conditions in thin lipid membrane stacks.

C-Laurdan is a modified Laurdan (HY-D0080) probe that imaging lipid rafts with environmentally sensitive fluorescence. C-Laurdan can be used in research of lipid arrangement and fluidity in biological membranes or artificial lipid membranes.

4-Methylumbelliferyl palmitate is an excellent fluorophore for measuring acid lipase in human leukocytes. Acidity and solvent have important influence on its fluorescence. 4-Methylumbelliferyl palmitate exists mainly as neutral molecular form which can be produced strong fluorescence at 445 nm in near neutral aqueous solutions, and exist mainly as anion form which can be produced stronger fluorescence at 445 nm in weak alkaline solutions.

25-NBD Cholesterol is a fluorescently labeled form of cholesterol that facilitates the distinction between exogenous and endogenous cholesterol within bacterial cell membranes, emitting green fluorescence signals.

3-C12-NBD-cholesterol is a fluorescent cholesterol analog that can be used for measuring membrane and intracellular trafficking dynamics.

DSPE-PEG1000-FITC is an amphiphile PEG polymer which has unsaturated lipid tails. The polymer can form micelles in an aqueous solution and can be used to prepare liposome/nano particles for drug nanocarriers. . Reagent grade, for research use only.

DSPE-PEG3400-FITC is a fluorescein attached PEG lipid. It can be used to prepare liposomes as drug carrier in targeted drug delivery. The polymer is modified with fluorescein (green) dye which can be used for staining cells, tissues, biomarkers, or nanoparticles.

C11 BODIPY 505/515 MG is a fluorescently labeled monoglyceride (Ex/Em = 495/503 nm) and can be used as a fluorescent probe.

C6-NBD Sphinganine is a sphinganine analog and can be used as fluorescent dye for labeling fatty acid.

18:1 PE AF 594 triammonium is a fluorescently labeled phospholipid probe. 18:1 PE AF 594 triammonium can be used as a fluorescent tracer for lipid nanoparticles (LNPs). 18:1 PE AF 594 triammonium is promising for research of drug delivery systems.

DSPE-PEG2000-FITC is a PEG lipid conjugated with fluorescein. FITC is a green dye with a peak absorption at 494 nm and a maximum emission at 520 nm, which is used for staining biological samples or nanoparticles. DSPE-PEG2000-FITC spontaneously forms lipid bilayers or micelles in water and can be used to prepare liposomes for delivering substances such as mRNA vaccines.

Cy7.5-PEG2000-DSPE is a PEG phospholipid with CY7.5 (HY-D0926) dye used in protein/nucelic acid labeling and fluorescence microscopy. Cy7.5-PEG2000-DSPE can self-assemble in aqueous solution to form micelles/lipid bilayer and used to prepare liposomes or nanoparticles for nutrients delivery such as mRNA or DNA vaccine (Ex/Em=770/820 nm).

DSPE-PEG5000-FITC is a fluorescein attached PEG lipid. It can be used to prepare liposomes as drug carrier in targeted drug delivery. The polymer is modified with fluorescein (green) dye which can be used for staining cells, tissues, biomarkers, or nanoparticles.

DSPE-PEG3400-Biotin is a phospholipid PEG for biotinylation. The amphiphilic property of the DSPE-PEG is useful for precision drug delivery and cancer therapy.

DSPE-PEG2000-Cy5 is a Cy5 (HY-D0821) fluorophore-labeled conjugate of distearoylphosphatidylethanolamine and polyethylene glycol, as well as a liposome component. The Cy5 fluorophore is commonly used for labeling proteins and nucleic acids in imaging, flow cytometry and genomic applications. DSPE-PEG2000-Cy5 supports cell membrane modification, in vivo tumor targeting research and long-term in vivo circulation of its liposomal formulations (Ex/Em=633/670 nm).

Sulforhodamine 101 DHPE is a fluorescent probe made from the conjugation of the phospholipid 1,2-dipalmitoyl-sn-glycero-3-PE to sulforhodamine 101, a red fluorescent dye that displays excitation/emission spectra of 586/605 nm, respectively. It integrates into phospholipid bilayers and has been used for imaging of solid supported lipid bilayers, detection of protein-ligand binding on bilayers, and to monitor colocalization of lipid probes in liposomes via resonance energy transfer (RET).

3-C6-NBD-cholesterol is a fluorescent analog of Chol that can be used to measure the kinetics of membrane and intracellular trafficking.

DSPE-PEG2000-Cy3 is a PEG lipid conjugated with a fluorophore. The Cy3 fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy and flow cytometry. The absorption wavelength of the Cy3 fluorophore peaks at 548-552 nm, while its emission wavelength reaches a maximum at 562-570 nm. DSPE-PEG2000-Cy3 can be used in research areas such as the preparation of nanoparticles and drug delivery.