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  3. Fluorescent Dye
  4. PE-Cy5

PE-Cy5 is a tandem fluorescent dye commonly used in flow cytometry, immunofluorescence, and cell biology research. It is formed by the covalent linkage of two fluorescent molecules, namely phycoerythrin and CY5. PE-Cy5 binds to human FcγRI (CD64), and this binding can be blocked by human pooled serum, anti-CD64 monoclonal antibodies targeting the ligand-binding region, or aggregated IgG. PE-Cy5 supports three-color flow cytometry analysis, and whole blood staining can partially reduce its non-specific binding (Ex/Em = 450-500 nm/665 nm).

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PE-Cy5

PE-Cy5 Chemical Structure

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PE-Cy5 Related Antibodies

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Description

PE-Cy5 is a tandem fluorescent dye commonly used in flow cytometry, immunofluorescence, and cell biology research. It is formed by the covalent linkage of two fluorescent molecules, namely phycoerythrin and CY5. PE-Cy5 binds to human FcγRI (CD64), and this binding can be blocked by human pooled serum, anti-CD64 monoclonal antibodies targeting the ligand-binding region, or aggregated IgG. PE-Cy5 supports three-color flow cytometry analysis, and whole blood staining can partially reduce its non-specific binding (Ex/Em = 450-500 nm/665 nm)[1][2].

In Vitro

PE-Cy5 conjugate specifically binds to cells expressing human FcγRI/CD64, including primary human monocytes, G-CSF-treated neutrophils, IFNγ-stimulated U937 cells, and FcγRI-transfected IIA1.6 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PE-Cy5 Related Antibodies
SMILES

[PE-Cy5]
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Purity & Documentation
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PE-Cy5 Related Classifications

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PE-Cy5Fluorescent Dyeacute myeloid leukemia-fab m3anti-CD64 monoclonal antibodiesG-CSF-treated patient neutrophilsIFNγ-stimulated U937 cellsprimary human monocyteshuman pooled serumaggregated IgGhuman peripheral blood mononuclear cellshuman FcγRI (CD64)FcγRI-transfected IIA1.6 cellsInhibitorinhibitorinhibit

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