Pinacyanol chloride
Pinacyanol chloride (Quinaldine blue) is a bright blue cyanine dye. Pinacyanol chloride is used for staining chromosomes in histology and for staining bacteria in biochemistry.
For research use only. We do not sell to patients.
- CAS No.: 2768-90-3
- Formula: C25H25ClN2
- Molecular Weight:388.93
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
Chemical Information
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CAS No. 2768-90-3
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Molecular Weight 388.93
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Formula C25H25ClN2
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SMILES
CCN1/C(C=CC2=C1C=CC=C2)=C/C=C/C3=CC=C4C=CC=CC4=[N+]3CC.[Cl-]
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Synonyms
Quinaldine blue
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Paraffin Section
The tissue is embedded in paraffin wax, cut into thin slices by a microtome, stained and sealed and finally made into samples for microscopic observation.
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Protocol for Hematoxylin-Eosin (H&E) Staining
Hematoxylin-eosin staining is a routine histological method that stains nuclei mainly blue-purple with hemalum and stains cytoplasm, extracellular matrix, and many stromal components pink with eosin, allowing tissue architecture, cell morphology, necrosis, inflammation, fibrosis, tumor growth pattern, and treatment-associated injury to be evaluated by light microscopy. In cancer cells, primary neurons, mouse tumor models, intestinal organoids, inflammatory macrophage preparations, and drug-screening tissues, H&E is a morphology assay rather than a molecular assay; it should be interpreted with complementary molecular or immunostaining assays when the biological question concerns specific proteins, RNA levels, ferroptosis, mitophagy, or immune phenotypes.
Purity & Documentation
References
[1]. Narayan RK. A technique for C-banding chromosomes with pinacyanol chloride. Stain Technol. 1980 Jan;55(1):9-11. [Content Brief]
[2]. Mitra A, et al. Studies on induction of metachromasy in cationic dye pinacyanol chloride by Klebsiella K7 capsular polysaccharide. Indian J Biochem Biophys. 1992 Oct;29(5):411-4. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)