1. Anti-infection
  2. Bacterial Antibiotic
  3. Polymyxin B nonapeptide TFA

Polymyxin B nonapeptide TFA is a cyclic peptide obtained from Polymyxin B by proteolytic removal of its terminal amino acyl residue. Polymyxin B nonapeptide TFA is less toxic, lacks bactericidal activity, and retains its ability to render gram-negative bacteria susceptible to several antibiotics by permeabilizing their outer membranes.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Polymyxin B nonapeptide TFA Chemical Structure

Polymyxin B nonapeptide TFA Chemical Structure

CAS No. : 2220175-42-6

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
USD 422 In-stock
Solution
10 mM * 1 mL in Water USD 422 In-stock
Solid
5 mg USD 150 In-stock
10 mg USD 250 In-stock
50 mg USD 800 In-stock
100 mg USD 1280 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Polymyxin B nonapeptide TFA:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Polymyxin B nonapeptide TFA

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Polymyxin B nonapeptide TFA is a cyclic peptide obtained from Polymyxin B by proteolytic removal of its terminal amino acyl residue. Polymyxin B nonapeptide TFA is less toxic, lacks bactericidal activity, and retains its ability to render gram-negative bacteria susceptible to several antibiotics by permeabilizing their outer membranes[1][2][3].

In Vitro

Polymyxin B nonapeptide TFA (2-32 μg/mL, 24 h) has a synergistic effect with Azithromycin (HY-17506)[1].
Polymyxin B nonapeptide TFA (10 μg/mL) inhibits  lipopolysaccharide (HY-D1056)-induced neutrophilic activation in a concentration-dependent manner[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: E. coli strains
Concentration: 2, 4, 8, 16, 32 μg/mL
Incubation Time: 24 h
Result: Combinated with AZT caused a significant reduction the bacterial growth after 6 h and complete killing after 24 h.
In Vivo

Polymyxin B nonapeptide TFA (1.5, 3.0 mg/kg intravenously for 29 days) does not exhibit neuromuscular blocking, neurotoxic, or nephrotoxic effects in rats as observed with polymyxin B sulfate[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1533.25

Formula

C43H74N14O11.5C2HF3O2

CAS No.
Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (65.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (32.61 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.6522 mL 3.2610 mL 6.5221 mL
5 mM 0.1304 mL 0.6522 mL 1.3044 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (65.22 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.82%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 0.6522 mL 3.2610 mL 6.5221 mL 16.3052 mL
5 mM 0.1304 mL 0.6522 mL 1.3044 mL 3.2610 mL
10 mM 0.0652 mL 0.3261 mL 0.6522 mL 1.6305 mL
15 mM 0.0435 mL 0.2174 mL 0.4348 mL 1.0870 mL
20 mM 0.0326 mL 0.1631 mL 0.3261 mL 0.8153 mL
25 mM 0.0261 mL 0.1304 mL 0.2609 mL 0.6522 mL
30 mM 0.0217 mL 0.1087 mL 0.2174 mL 0.5435 mL
DMSO 40 mM 0.0163 mL 0.0815 mL 0.1631 mL 0.4076 mL
50 mM 0.0130 mL 0.0652 mL 0.1304 mL 0.3261 mL
60 mM 0.0109 mL 0.0544 mL 0.1087 mL 0.2718 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Polymyxin B nonapeptide TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Polymyxin B nonapeptide TFA
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