1. Anti-infection
    Autophagy
  2. Bacterial
    Autophagy
    Antibiotic
  3. Azithromycin

Azithromycin (Synonyms: CP 62993)

Cat. No.: HY-17506 Purity: >98.0%
Handling Instructions

Azithromycin is a macrolide antibiotic useful for the treatment of a number of bacterial infections.

For research use only. We do not sell to patients.

Azithromycin Chemical Structure

Azithromycin Chemical Structure

CAS No. : 83905-01-5

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10 mM * 1 mL in DMSO USD 66 In-stock
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50 mg USD 60 In-stock
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100 mg USD 96 In-stock
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200 mg USD 144 In-stock
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Azithromycin:

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Description

Azithromycin is a macrolide antibiotic useful for the treatment of a number of bacterial infections.

In Vitro

Azithromycin (2 μM) augments rhinovirus-induced IFNβ expression in primary bronchial epithelial cells from asthmatics, which is associated with over-expression of RIG-I like receptors and repression of viral replication. Knockdown of MDA5, but not knockdown of RIG-I, diminishes azithromycin (2 μM)-enhanced viral-induced IFNβ expression in asthmatic primary bronchial epithelial cells[1]. Azithromycin specifically reduces MMP-9 mRNA and protein levels without affecting NF-κB in endotoxin-challenged monocytic THP-1 cells[2].

In Vivo

Azithromycin (50 mg/kg) has no effect on bronchoalveolar lavage inflammatory parameters and LDH levels in a mouse model of asthma exacerbation. Azithromycin induces neither general inflammatory parameters nor LDH release in a mouse model of asthma exacerbation, and augments expression of interferon-stimulated genes and the pattern recognition receptor MDA5 but not RIG-I in exacerbating mice[1].

Clinical Trial
Molecular Weight

748.98

Formula

C₃₈H₇₂N₂O₁₂

CAS No.

83905-01-5

SMILES
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (133.51 mM)

H2O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3351 mL 6.6757 mL 13.3515 mL
5 mM 0.2670 mL 1.3351 mL 2.6703 mL
10 mM 0.1335 mL 0.6676 mL 1.3351 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (3.34 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (3.34 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (3.34 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[2]

THP-1 cells (106 cells in 1 mL RPMI medium, without antibiotics, growth factors or serum) are seeded in each well of 24-well plates and allowed to settle for 1 hour. Next, 50 μL of the test compound is added followed by 50 μL of LPS (final concentration of 10 μg/mL). After 24h (37°C and 5% CO2) the supernatants and cell pellets are collected (1200 rpm, 5 min). THP-1 cell viability is tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). MTT is dissolved at 2 mg/mL in PBS and aliquots are stored at -20°C. The MTT assay is performed according to the suppliers instructions. Absorbance of MTT converted into formazan is measured at a wavelength of 570 nm with background subtraction at 630 nm.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: >98.0%

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Keywords:

AzithromycinCP 62993CP62993CP-62993BacterialAutophagyAntibioticInhibitorinhibitorinhibit

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Product Name:
Azithromycin
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HY-17506
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