CDC42BPG/DMPK2

CDC42BPG, also referred to as DMPK2 or MRCK gamma, is the least characterised of the three CDC42-binding kinases and lacks much of the extended coiled-coil tail carried by MRCK alpha and MRCK beta. It retains the DMPK-family kinase core and the N-terminal CRIB-like CDC42/RAC-binding motif, and it phosphorylates myosin light chain in vitro, yet its cellular functions remain poorly defined, with reported roles in cytoskeletal remodelling and localisation to the centrosome and midbody during cell division. Given this limited literature, recombinant CDC42BPG is used mainly for kinase-panel profiling, isoform-selectivity screening against MRCK alpha, MRCK beta and ROCK1/2, and biochemical characterisation of the subfamily.