Novel bioactive clerodane diterpenoids from the leaves and twigs of Casearia sylvestris

  • J Nat Prod. 2002 Feb;65(2):95-9. doi: 10.1021/np010459m.
Nicholas H Oberlies  1 ,  Jason P Burgess ,  Hernán A Navarro ,  Rosa Elena Pinos ,  Craig R Fairchild ,  Russell W Peterson ,  Djaja D Soejarto ,  Norman R Farnsworth ,  A Douglas Kinghorn ,  Mansukh C Wani ,  Monroe E Wall
Affiliations
  • 1. Chemistry and Life Sciences, Research Triangle Institute, P.O. Box 12194, Research Triangle Park, North Carolina 27709-2194, USA.
PMID: 11858737 DOI: 10.1021/np010459m
Abstract

Fractionation of a methanol extract of the leaves and twigs of Casearia sylvestris, as directed by activity against KB cell cytotoxicity, led to the isolation of three novel clerodane Diterpenoids, casearvestrins A-C (1-3). The structures of 1-3 were deduced from one- and two-dimensional NMR experiments, including relative stereochemical assignments based on ROESY correlations and COSY coupling constants. All three compounds displayed promising bioactivity, both in cytotoxicity assays against a panel of tumor cell lines and in Antifungal assays via the growth inhibition of Aspergillus niger in a disk diffusion assay.