Stachyflin and acetylstachyflin, novel anti-influenza A virus substances, produced by Stachybotrys sp. RF-7260. I. Isolation, structure elucidation and biological activities

  • J Antibiot (Tokyo). 2002 Feb;55(2):155-64. doi: 10.7164/antibiotics.55.155.
Kazuyuki Minagawa  1 ,  Shuichi Kouzuki ,  Jun Yoshimoto ,  Yoshimi Kawamura ,  Hiroyoshi Tani ,  Tatsuo Iwata ,  Yoshihiro Terui ,  Hiroshi Nakai ,  Shigenori Yagi ,  Naohiko Hattori ,  Tamio Fujiwara ,  Toshiyuki Kamigauchi
Affiliations
PMID: 12002997 DOI: 10.7164/antibiotics.55.155
Abstract

Two novel compounds, stachyflin and acetylstachyflin, have been isolated by solid-state fermentation of Stachybotrys sp. RF-7260. The structures of both metabolites, determined by detailed NMR analyses and X-ray crystallographic analysis, are novel with a pentacyclic moiety including cis-fused decalin. The absolute stereochemistry of stachyflins was determined by circular dichroism analysis. Stachyflin showed Antiviral activity against influenza A virus (H1N1) in vitro with an IC50 value of 0.003 microM. Acetylstachyflin was about 77-fold less active than stachyflin.

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