Demonstration of a neurokinin A receptor subtype in transfected fibroblasts

  • Eur J Pharmacol. 1992 Feb 13;225(2):175-8. doi: 10.1016/0922-4106(92)90099-h.
A K Henderson  1 ,  H I Yamamura ,  C A Maggi ,  S H Buck ,  P L Van Giersbergen ,  W R Roeske
Affiliations
  • 1. Department of Pharmacology, University of Arizona, College of Medicine, Tucson 85724.
PMID: 1312943 DOI: 10.1016/0922-4106(92)90099-h
Abstract

In competitive radioligand binding assays, the NK2 receptor antagonists [Tyr5,D-Trp6,8,9,Arg10]NKA(4-10) (MEN 10207) and [Tyr5,D-Trp6,8,9,Arg10]NKA(3-10) (MEN 10208) had high and low affinity, respectively, in bovine stomach membranes and SKLKB82#3 cells, a murine fibroblast cell line transfected with a cDNA encoding for the bovine NK2 receptor. These antagonists also had different affinities when inhibiting neurokinin A-induced polyphosphoinositide hydrolysis in SKLKB82#3 murine fibroblasts. Thus, the de novo protein expressed by the SKLKB82#3 murine fibroblasts may represent a distinct NK2 receptor subtype.

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