KIAA0649, a 1A6/DRIM-interacting protein with the oncogenic potential

  • Biochem Biophys Res Commun. 2005 Sep 2;334(3):884-90. doi: 10.1016/j.bbrc.2005.06.179.
Lin Yang  1 ,  Jun Zhao ,  Wenqing Lü ,  Yong Li ,  Xiaojuan Du ,  Tao Ning ,  Guirong Lu ,  Yang Ke
Affiliations
  • 1. Beijing Institute for Cancer Research, School of Oncology, Peking University No. 1 Da Hong Luo Chang St, West District, Beijing 100034, PR China.
PMID: 16053918 DOI: 10.1016/j.bbrc.2005.06.179
Abstract

We identified a 1A6/DRIM (down-regulated in metastasis) interacting protein, KIAA0649 during the yeast two-hybrid screen. The interaction between KIAA0649 and 1A6/DRIM was further confirmed by GST-pull-down and co-immunoprecipitation assays. KIAA0649 was originally identified from human brain tissue. However, its biological function remains unknown. In this report, we showed that KIAA0649 mRNA is widely expressed in human multiple tissues and cell lines. We have also demonstrated that KIAA0649 has oncogenic characteristics: it enhances colony formation, allows anchorage-independent growth. Moreover, KIAA0649 exogenous expression in NIH3T3 fibroblasts caused tumor development in nude mice.