Growth and drug resistance phenotypes resulting from cytomegalovirus DNA polymerase region III mutations observed in clinical specimens

  • Antimicrob Agents Chemother. 2007 Nov;51(11):4160-2. doi: 10.1128/AAC.00736-07.
Sunwen Chou  1 ,  Gail I Marousek ,  Laura C Van Wechel ,  Shaobing Li ,  Adriana Weinberg
Affiliations
  • 1. Divisionof Infectious Disease, Oregon Health Science University, VA Medical Center, Portland, OR 97239, USA. [email protected]
PMID: 17709468 DOI: 10.1128/AAC.00736-07
Abstract

Recombinant phenotyping of cytomegalovirus (CMV) pol region III mutations from clinical specimens showed that T813S and G841A each conferred foscarnet resistance and approximately threefold increased ganciclovir resistance; adding the UL97 mutation C592G increased ganciclovir resistance to approximately sixfold. Bacterial artificial chromosome CMV clones containing pol mutation L845P were nonviable unless repaired with the wild-type sequence.