Synthesis of nucleotide analogues by a promiscuous phosphoribosyltransferase

  • Org Lett. 2007 Oct 11;9(21):4179-82. doi: 10.1021/ol7016802.
Robert A Scism  1 ,  Donald F Stec ,  Brian O Bachmann
Affiliations
  • 1. Department of Chemistry, Vanderbilt University, 7300 Stevenson Center Lane, Nashville, Tennessee 37235, USA.
PMID: 17854193 DOI: 10.1021/ol7016802
Abstract

An Escherichia coli strain overexpressing a mutant variant of a phosphoribosyl transferase was developed as a catalyst for the efficient preparation of a range of purine nucleotide analogues. This system offers an efficient and rapid method for nucleotide analogue synthesis with 100% beta-selectivity, providing analytically pure product in a single purification step.

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