Delivery and visualization of proteins conjugated to quantum dots in cardiac myocytes

  • J Mol Cell Cardiol. 2008 Dec;45(6):853-6. doi: 10.1016/j.yjmcc.2008.08.006.
Yevgeniya E Koshman  1 ,  Stephen B Waters ,  Lori A Walker ,  Tamara Los ,  Pieter de Tombe ,  Paul H Goldspink ,  Brenda Russell
Affiliations
  • 1. Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, USA.
PMID: 18835396 DOI: 10.1016/j.yjmcc.2008.08.006
Abstract

The design of a novel transduction complex has permitted the introduction of protein-quantum dot conjugates into the cytoplasm of living cells. Appropriate subcellular localization of quantum dot-conjugated cardiac troponin C to the myofibrils and a nuclear peptide to the nucleus was attained in living cardiac myocytes using this approach. This new methodology opens the possibility for live tracking of exogenous proteins and study of protein dynamics.

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