Antiprotealide is a natural product

  • J Nat Prod. 2009 Feb 27;72(2):295-7. doi: 10.1021/np800578e.
Rama Rao Manam  1 ,  Venkat R Macherla ,  Ginger Tsueng ,  Chris W Dring ,  Jeffrey Weiss ,  Saskia T C Neuteboom ,  Kin S Lam ,  Barbara C Potts
Affiliations
  • 1. Nereus Pharmaceuticals, Inc, 10480 Wateridge Circle, San Diego, California 92121, USA.
PMID: 19133779 DOI: 10.1021/np800578e
Abstract

Large-scale fermentation of the marine actinomycete Salinispora tropica for production of salinosporamide A (NPI-0052; 1) clinical trials Materials provided crude extracts containing minor secondary metabolites, including salinosporamide B (2) and a new congener, 3. Spectroscopic characterization revealed that 3 is identical to antiprotealide, a molecular hybrid of 20S Proteasome inhibitors 1 and omuralide (4) not previously described as a natural product. Analysis of crude extracts from shake flask cultures of three wild-type S. tropica strains confirmed the production of antiprotealide at 1.1, 0.8, and 3.0 mg/L. Thus, antiprotealide is a natural product metabolite of S. tropica.