Co-immunoprecipitation and protein blots

  • Methods Mol Biol. 2010:655:377-87. doi: 10.1007/978-1-60761-765-5_25.
Erika Isono  1 ,  Claus Schwechheimer
Affiliations
  • 1. Department of Plant Systems Biology, Technical University Munich-Weihenstephan, Munich, Germany.
PMID: 20734274 DOI: 10.1007/978-1-60761-765-5_25
Abstract

Knowledge about the identity of the interacting partners is important for the understanding of the function and the cellular activity of a given protein. Here we describe co-immunoprecipitation and pull-down as methods that are widely used for the identification and characterization of protein-protein interactions. These methods are well suited to find or confirm the interaction among multiple proteins, given the availability of a specific antibody for or a tagged version of the protein of interest.