Dafadine inhibits DAF-9 to promote dauer formation and longevity of Caenorhabditis elegans

  • Nat Chem Biol. 2011 Nov 6;7(12):891-3. doi: 10.1038/nchembio.698.
Genna M Luciani  1 ,  Lilia Magomedova ,  Rachel Puckrin ,  Malene L Urbanus ,  Iain M Wallace ,  Guri Giaever ,  Corey Nislow ,  Carolyn L Cummins ,  Peter J Roy
Affiliations
  • 1. Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
PMID: 22057127 DOI: 10.1038/nchembio.698
Abstract

The DAF-9 Cytochrome P450 is a key regulator of dauer formation, developmental timing and longevity in the nematode Caenorhabditis elegans. Here we describe the first identified chemical inhibitor of DAF-9 and the first reported small-molecule tool that robustly induces dauer formation in typical culture conditions. This molecule (called dafadine) also inhibits the mammalian ortholog of DAF-9(CYP27A1), suggesting that dafadine can be used to interrogate developmental control and longevity in other Animals.

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